ZFIN ID: ZDB-PUB-090311-1
Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer
Chen, B., Dodge, M.E., Tang, W., Lu, J., Ma, Z., Fan, C.W., Wei, S., Hao, W., Kilgore, J., Williams, N.S., Roth, M.G., Amatruda, J.F., Chen, C., and Lum, L.
Date: 2009
Source: Nature Chemical Biology   5(2): 100-107 (Journal)
Registered Authors: Amatruda, James F.
Keywords: none
MeSH Terms:
  • Axin Protein
  • Humans
  • Molecular Structure
  • Neoplasms/metabolism*
  • Regeneration*
  • Repressor Proteins/metabolism
  • Signal Transduction/drug effects*
  • Signal Transduction/physiology
  • Wnt Proteins/metabolism
  • Wnt Proteins/physiology*
  • beta Catenin/metabolism
  • beta Catenin/physiology
PubMed: 19125156 Full text @ Nat. Chem. Biol.
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ABSTRACT
The pervasive influence of secreted Wnt signaling proteins in tissue homeostasis and tumorigenesis has galvanized efforts to identify small molecules that target Wnt-mediated cellular responses. By screening a diverse synthetic chemical library, we have discovered two new classes of small molecules that disrupt Wnt pathway responses; whereas one class inhibits the activity of Porcupine, a membrane-bound acyltransferase that is essential to the production of Wnt proteins, the other abrogates destruction of Axin proteins, which are suppressors of Wnt/beta-catenin pathway activity. With these small molecules, we establish a chemical genetic approach for studying Wnt pathway responses and stem cell function in adult tissue. We achieve transient, reversible suppression of Wnt/beta-catenin pathway response in vivo, and we establish a mechanism-based approach to target cancerous cell growth. The signal transduction mechanisms shown here to be chemically tractable additionally contribute to Wnt-independent signal transduction pathways and thus could be broadly exploited for chemical genetics and therapeutic goals.
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