ZFIN ID: ZDB-PUB-090309-3
Exploring Differential Gene Expression in Zebrafish to Teach Basic Molecular Biology Skills
Schmoldt, A., Forecki, J., Hammond, D.R., and Udvadia, A.J.
Date: 2009
Source: Zebrafish   6(2): 187-199 (Other)
Registered Authors: Schmoldt, Angela, Udvadia, Ava J.
Keywords: none
MeSH Terms:
  • Animals
  • Clinical Laboratory Techniques
  • Gene Expression Regulation*
  • Molecular Biology/education*
  • Universities
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 19250030 Full text @ Zebrafish
In an effort to engage students in original research while teaching them basic molecular biology skills, we have designed a course for upper level undergraduate students and beginning graduate students that employs in situ hybridization in whole-mount zebrafish embryos to explore the concept of differential gene regulation. The course was taught in a workshop format during a break between the normal fall and spring semesters, which allowed students to immerse themselves in the concepts and techniques full time over a 13-day period. Overall, the course was successful in exposing students to a variety of techniques in the context of an ongoing research project in our laboratory, which provided beneficial outcomes for students and instructors alike. Here we provide a detailed account of the course organization and preparation, as well as an analysis of learning outcomes achieved by the students.