PUBLICATION
Acute and Chronic alcohol dose: Population differences in behavior and neurochemistry of zebrafish
- Authors
- Gerlai, R., Chatterjee, D., Pereira, T., Sawashima, T., and Krishnannair, R.
- ID
- ZDB-PUB-090302-26
- Date
- 2009
- Source
- Genes, brain, and behavior 8(6): 586-599 (Journal)
- Registered Authors
- Gerlai, Robert T.
- Keywords
- none
- MeSH Terms
-
- Acute Disease
- Alcohol-Related Disorders/genetics
- Alcohol-Related Disorders/metabolism
- Alcohol-Related Disorders/physiopathology
- Animals
- Behavior, Animal/drug effects*
- Behavior, Animal/physiology
- Brain Chemistry/drug effects*
- Brain Chemistry/genetics
- Chronic Disease
- Disease Models, Animal
- Ethanol/administration & dosage*
- Female
- Male
- Zebrafish/genetics
- Zebrafish/metabolism*
- PubMed
- 19243447 Full text @ Genes Brain Behav.
Citation
Gerlai, R., Chatterjee, D., Pereira, T., Sawashima, T., and Krishnannair, R. (2009) Acute and Chronic alcohol dose: Population differences in behavior and neurochemistry of zebrafish. Genes, brain, and behavior. 8(6):586-599.
Abstract
The zebrafish has been in the forefront of developmental genetics for decades and has also been gaining attention in neurobehavioral genetics. It has been proposed to model alcohol induced changes in human brain function and behavior. Here, adult zebrafish populations, AB and SF (short fin wild type), were exposed to chronic treatment (several days in 0.00% or 0.50% alcohol vol/vol) and a subsequent acute treatment (one hour in 0.00, 0.25, 0.50 or 1.00 % alcohol). Behavioral responses of zebrafish to computer animated images, including a zebrafish shoal and a predator, were quantified using videotracking. Neurochemical changes in the dopaminergic and serotoninergic systems in the brain of the fish were measured using high precision liquid chromatography (HPLC) with electrochemical detection. The results demonstrated genetic differences in numerous aspects of alcohol induced changes including, for the first time, in the behavioral effects of withdrawal from alcohol and in neurochemical responses to alcohol. For example, withdrawal from alcohol abolished shoaling and increased dopamine and DOPAC in AB but not in SF fish. The findings show that, first, acute and chronic alcohol induced changes are quantifiable with automated behavioral paradigms; second, robust neurochemical changes are also detectable; and third, genetic factors influence both alcohol induced behavioral and neurotransmitter level changes. Although the causal relationship underlying the alcohol induced changes in behavior and neurochemistry is speculative at this point, the results suggest that zebrafish will be a useful tool for the analysis of the biological mechanisms of alcohol induced functional changes in the adult brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping