ZFIN ID: ZDB-PUB-090217-5
The transcription factor, glial cell missing 2, is involved in differentiation and functional regulation of H+-ATPase-rich cells in zebrafish (Danio rerio)
Chang, W.J., Horng, J.L., Yan, J.J., Hsiao, C.D., and Hwang, P.P.
Date: 2009
Source: American journal of physiology. Regulatory, integrative and comparative physiology   296(4): R1192-R1201 (Journal)
Registered Authors: Horng, Jiun-Lin, Hsiao, Chung-Der, Hwang, Pung Pung
Keywords: none
MeSH Terms:
  • Acid-Base Equilibrium*
  • Adaptation, Physiological
  • Animals
  • Cell Differentiation*
  • Cloning, Molecular
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism*
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Knockdown Techniques
  • Gills/embryology
  • Gills/enzymology*
  • In Situ Hybridization
  • RNA, Messenger/metabolism
  • Skin/embryology
  • Skin/enzymology*
  • Sodium-Potassium-Exchanging ATPase/metabolism
  • Time Factors
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Vacuolar Proton-Translocating ATPases/genetics
  • Vacuolar Proton-Translocating ATPases/metabolism*
  • Zebrafish/embryology
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 19193938 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.
H(+)-ATPase-rich (HR) cells in zebrafish are known to be involved in acid secretion and Na(+) uptake mechanisms in zebrafish gills/skin; however, little is known about how HR cells are functionally regulated. In the present work, we studied the roles of Drosophila glial cell missing (gcm), a cell fate-related transcription factor, in the differentiation and functional regulation of zebrafish HR cells. Zebrafish gcm-2 (zgcm2) was found to begin expression in zebrafish embryos at 10 h post-fertilization (hpf), and to be extensively expressed in gills but only mildly so in eyes, heart, muscles, and testes. By whole-mount in situ hybridization, zgcm2 messenger (m)RNA signals were found in a group of cells on the zebrafish yolk-sac surface initially in the tail-bud stage (10 hpf); they had disappeared at 36 hpf, and thereafter appeared again in the gill region from 48 hpf. Double fluorescence in situ hybridization further demonstrated specific co-localization of zgcm2 mRNA in HR cells in zebrafish embryons. Knockdown of zgcm2 with a specific morpholino oligonucleotide caused the complete disappearance of HR cells with a concomitant decrease in H(+) activity at the apical surface of HR cells, but it did not affect the occurrence of Na(+)-K(+)-ATPase-rich (NaR)cells. A decrease in zatp6v1a (the H(+)-ATPase subunit A) expression and no change in zgcm2 expression in zebrafish gills were seen from 12 h to 3 d after transfer to acidic fresh water, but a compensatory stimulation in the expressions of both genes appeared 4 d post transfer. In conclusion, functional regulation of HR cells is probably achieved by enhancing cell differentiation via zGCM2 activation. Key words: acid secretion, ionocytes, ion regulation, gills.