|ZFIN ID: ZDB-PUB-090116-21|
Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration
Liongue, C., Hall, C.J., O'Connell, B.A., Crosier, P., and Ward, A.C.
|Source:||Blood 113(11): 2535-2546 (Journal)|
|Registered Authors:||Crosier, Phil, Hall, Chris, Liongue, Clifford, Ward, Alister C.|
|PubMed:||19139076 Full text @ Blood|
Liongue, C., Hall, C.J., O'Connell, B.A., Crosier, P., and Ward, A.C. (2009) Zebrafish granulocyte colony-stimulating factor receptor signaling promotes myelopoiesis and myeloid cell migration. Blood. 113(11):2535-2546.
ABSTRACTGranulocyte colony-stimulating factor receptor (GCSFR) signaling participates in the production of neutrophilic granulocytes during normal hematopoietic development, with a particularly important role during emergency hematopoiesis. This study describes the characterization of the zebrafish gcsf and gcsfr genes, which showed broad conservation and similar regulation to their mammalian counterparts. Morpholino-mediated knockdown of gcsfr and overexpression of gcsf revealed the presence of an anterior population of myeloid cells during primitive hematopoiesis that were dependent on GCSF/GCSFR for their development and migration. This contrasted with a posterior domain that was largely independent of this pathway. Definitive myelopoiesis was also partially dependent on a functional GCSF/GCSFR pathway. Injection of bacterial lipopolysaccharide elicited significant induction of gcsf expression and emergency production of myeloid cells, which was abrogated by gcsfr knockdown. Collectively, these data demonstrate GCSF/GCSFR to be a conserved signaling system for facilitating the production of multiple myeloid cell lineages in both homeostatic and emergency conditions, as well as for early myeloid cell migration, establishing a useful experimental platform for further dissection of this pathway.