ZFIN ID: ZDB-PUB-090116-20
Effects of arsenic (As) exposure on the antioxidant status of gills of the zebrafish Danio rerio (Cypridinae)
Ventura-Lima, J., de Castro, M.R., Acosta, D., Fattorini, D., Regoli, F., de Carvalho, L.M., Bohrer, D., Geracitano, L.A., Barros, D.M., Marins, L.F., da Silva, R.S., Bonan, C.D., Bogo, M.R., and Monserrat, J.M.
Date: 2009
Source: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP   149(4): 538-543 (Journal)
Registered Authors: Marins, Luis Fernando
Keywords: Antioxidant defenses, Gills, Glutathione, Zebrafish, Arsenic
MeSH Terms:
  • Animals
  • Antioxidants/metabolism*
  • Arsenic/metabolism
  • Arsenic/toxicity*
  • Gills/drug effects
  • Gills/metabolism*
  • Glutathione/metabolism
  • Glutathione Reductase/metabolism
  • Glutathione Transferase/metabolism
  • Lipid Peroxidation
  • Oxygen Consumption/drug effects
  • Zebrafish/metabolism*
PubMed: 19138757 Full text @ Comp. Biochem. Physiol. C Toxicol. Pharmacol.
In fishes, arsenic (As) is absorbed via the gills and is capable of causing disturbance to the antioxidant system. The objective of present study was to evaluate antioxidant responses after As exposure in gills of zebrafish (Danio rerio, Cypridinae). Fish were exposed for 48 h to three concentration of As, including the highest As concentration allowed by current Brazilian legislation (10 mug As/L). A control group was exposed to tap water (pH 8.0; 26 degrees C; 7.20 mg O(2)/L). As exposure resulted in (1) an increase (p<0.05) of glutathione (GSH) levels after exposure to 10 and 100 mug As/L, (2) an increase of the glutamate cysteine ligase (GCL) activity in the same concentrations (p<0.05), (3) no significant differences in terms of glutathione reductase, glutathione-S-transferase and catalase activities; (4) a significantly lower (p<0.05) oxygen consumption after exposure to 100 mug As/L; (4) no differences in terms of oxygen reactive species generation and lipid peroxidation content (p>0,05). In the gills, only inorganic As was detected. Overall, it can be concluded that As affected the antioxidant responses increasing GCL activity and GSH levels, even at concentration considered safe by Brazilian legislation.