PUBLICATION
Image analysis of calcium release dynamics
- Authors
- Freisinger, C.M., Houston, D.W., and Slusarski, D.C.
- ID
- ZDB-PUB-090106-7
- Date
- 2008
- Source
- Methods in molecular biology (Clifton, N.J.) 468: 145-156 (Chapter)
- Registered Authors
- Slusarski, Diane C.
- Keywords
- Calcium, Wnt-5, Zebrafish, Xenopus, Fura-2, Wnt/calcium, Wnt/beta-catenin, Ratiometric calcium imaging
- MeSH Terms
-
- Animals
- Calcium/metabolism*
- Calcium Signaling/physiology*
- Image Processing, Computer-Assisted*
- Microinjections
- Wnt Proteins/metabolism
- Zebrafish
- PubMed
- 19099252 Full text @ Meth. Mol. Biol.
Citation
Freisinger, C.M., Houston, D.W., and Slusarski, D.C. (2008) Image analysis of calcium release dynamics. Methods in molecular biology (Clifton, N.J.). 468:145-156.
Abstract
Many aspects of animal development are dependent on the dynamic release of calcium (Ca2+) ions. Although Ca2+ release within a cell is tightly controlled, how the release dynamics result in a specific biological output in embryonic development is less clear. The integration of pharmacological and molecular-genetic studies with in vivo imaging in zebrafish and Xenopus has linked endogenous Ca2+ release to the Wnt signaling network. Our data suggests that distinct Ca2+ release dynamics lead to antagonism of the developmentally important Wnt/beta-catenin pathway while sustained Ca2+ release modulates polarized cell movements.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping