|ZFIN ID: ZDB-PUB-081218-36|
Distribution of cytoskeletal and adhesion proteins in adult zebrafish skeletal muscle
Câmara-Pereira, E.S., Campos, L.M., Vannier-Santos, M.A., Mermelstein, C.S., and Costa, M.L.
|Source:||Histology and histopathology 24(2): 187-196 (Journal)|
|Registered Authors:||Costa, Manoel Luis, Mermelstein, Claudia|
|Keywords:||Muscle, Zebrafish, Cytoskeleton, Extracellular matrix, Cell adhesion|
Câmara-Pereira, E.S., Campos, L.M., Vannier-Santos, M.A., Mermelstein, C.S., and Costa, M.L. (2009) Distribution of cytoskeletal and adhesion proteins in adult zebrafish skeletal muscle. Histology and histopathology. 24(2):187-196.
ABSTRACTThe organization of cytoskeletal and adhesion proteins in skeletal muscle is critical for its contractile function. Zebrafish has become a paramount model for studies of vertebrate biology, including muscle. However, only a few studies have been published using immunolabeling to specifically localize proteins in adult zebrafish muscle. To fully appreciate the distribution of cytoskeletal and adhesion proteins, and therefore to better correlate the adult muscle with its myogenesis, we used indirect immunofluorescence microscopy of frozen adult zebrafish skeletal muscle sections. Here we describe the fish muscle cytoskeletal architecture and location of the major myofibrillar proteins desmin, alpha-actinin, myosin, titin, troponin, tropomyosin and nebulin, the adhesion proteins vinculin and paxillin, and the extracellular matrix proteins laminin and fibronectin. Electron microscopical analysis in ultra-thin sections of adult zebrafish skeletal muscle showed bundles of collagen fibers and fibroblastic cells in the extracellular space of the myosepta.