|ZFIN ID: ZDB-PUB-081218-20|
The Sphingolipid Transporter Spns2 Functions in Migration of Zebrafish Myocardial Precursors
Kawahara, A., Nishi, T., Hisano, Y., Fukui, H., Yamaguchi, A., and Mochizuki, N.
|Source:||Science (New York, N.Y.) 323(5913): 524-527 (Journal)|
|Registered Authors:||Kawahara, Atsuo|
|PubMed:||19074308 Full text @ Science|
Kawahara, A., Nishi, T., Hisano, Y., Fukui, H., Yamaguchi, A., and Mochizuki, N. (2009) The Sphingolipid Transporter Spns2 Functions in Migration of Zebrafish Myocardial Precursors. Science (New York, N.Y.). 323(5913):524-527.
ABSTRACTSphingosine-1-phosphate (S1P) is a secreted lipid mediator that functions in vascular development: however, it remains unclear how S1P secretion is regulated during embryogenesis. We identified a zebrafish mutant ko157, which displays cardia bifida (two hearts) resembling the S1P receptor-2 mutant. A migration defect of myocardial precursors in the ko157 mutant is due to a mutation in a multipass transmembrane protein Spns2 and can be rescued by S1P injection. We show that the export of S1P from cells requires Spns2. spns2 is expressed in the extra-embryonic tissue yolk syncytial layer (YSL) and introduction of spns2 mRNA in the YSL restored the cardiac defect in the ko157 mutant. Thus, Spns2 in the YSL functions as a S1P transporter in S1P secretion, thereby regulating myocardial precursor migration.