PUBLICATION
The aqueous humor outflow pathway of zebrafish
- Authors
- Gray, M.P., Smith, R.S., Soules, K.A., John, S.W., and Link, B.
- ID
- ZDB-PUB-081217-18
- Date
- 2009
- Source
- Investigative ophthalmology & visual science 50(4): 1515-1521 (Journal)
- Registered Authors
- Link, Brian, Soules, Kelly
- Keywords
- aqueous flow, electron microscopy, trabecular meshwork
- MeSH Terms
-
- Absorption
- Animals
- Aqueous Humor
- Biological Transport, Active/physiology
- Choroid/blood supply*
- Choroid/ultrastructure
- Ciliary Body/metabolism*
- Ciliary Body/ultrastructure
- Ligaments
- Microscopy, Electron, Transmission
- Microscopy, Fluorescence
- Trabecular Meshwork/metabolism
- Veins/physiology
- Zebrafish/physiology*
- PubMed
- 19060283 Full text @ Invest. Ophthalmol. Vis. Sci.
Citation
Gray, M.P., Smith, R.S., Soules, K.A., John, S.W., and Link, B. (2009) The aqueous humor outflow pathway of zebrafish. Investigative ophthalmology & visual science. 50(4):1515-1521.
Abstract
PURPOSE. The structures of the ocular anterior segment responsible for aqueous humor secretion and absorption have been well characterized in mammals. However, the underlying molecular and cellular mechanisms that regulate aqueous humor flow have remained elusive. Experimental analysis in Danio rerio, the zebrafish, is providing mechanistic insights into many cellular processes relevant to normal human physiology and disease. To facilitate studies on the molecular and cellular mechanisms of aqueous humor dynamics using this species, we have characterized the anatomy of aqueous secretion and outflow in adult zebrafish eyes. METHODS. Analysis by light and transmission electron microscopy, coupled with molecular tracers of fluid flow, was employed to identify and study the sites of aqueous humor secretion and absorption in adult zebrafish eyes. RESULTS. Zebrafish eyes show aqueous humor secretion primarily from the dorsal ciliary region and outflow through a ventral canalicular network that connects with an aqueous plexus and veins of the choroidal rete. CONCLUSIONS. Vectorial flow of zebrafish aqueous humor is in contrast to mammals where secretion and absorption of aqueous humor is circumferential around and through the iridocorneal angle. However, local anatomy and ultrastructure of the tissues and cells specialized for aqueous humor dynamics in zebrafish shows conservation with that of mammals. These observations suggest that zebrafish can serve as a useful genetic model to help understand the regulation and cellular basis of normal and abnormal aqueous humor dynamics in humans.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping