PUBLICATION
Reverse genetics in zebrafish by TILLING
- Authors
- Moens, C.B., Donn, T.M., Wolf-Saxon, E.R., and Ma, T.P.
- ID
- ZDB-PUB-081203-8
- Date
- 2008
- Source
- Briefings in functional genomics & proteomics 7(6): 454-459 (Review)
- Registered Authors
- Donn, Thomas, Moens, Cecilia
- Keywords
- zebrafish, TILLING, Cel1 mismatch cleavage, resequencing, reverse genetics
- MeSH Terms
-
- Animals
- Base Pair Mismatch
- Ethylnitrosourea/administration & dosage
- Models, Biological
- Mutagenesis
- Mutagens/administration & dosage
- Zebrafish/genetics*
- PubMed
- 19028802 Full text @ Brief. Funct. Genomic. Proteomic.
Citation
Moens, C.B., Donn, T.M., Wolf-Saxon, E.R., and Ma, T.P. (2008) Reverse genetics in zebrafish by TILLING. Briefings in functional genomics & proteomics. 7(6):454-459.
Abstract
TILLING, for Targeting Induced Local Lesions in Genomes, is a reverse genetics strategy that identifies mutations in specific genes of interest in chemically mutagenized populations. First described in 2000 for mutation detection in Arabidopsis, TILLING is now used in a wide range of plants including soybean, rice, barley and maize as well as for animal model systems, including Arabidopsis, Drosophila, Caenorhabditis elegans, rat, medaka and zebrafish and for the discovery of naturally occurring polymorphisms in humans. This review summarizes current TILLING methodologies as they have been applied to the zebrafish, ongoing TILLING projects and resources in the zebrafish community, and the future of zebrafish TILLING.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping