header logo image header logo text
Downloads Login
General Information
ZFIN ID: ZDB-PUB-081114-23
Transcriptome analysis of the zebrafish mind bomb mutant
Hwang, J., Kim, H.S., Seok, J.W., Kim, J.D., Koun, S., Park, S.Y., Lee, J., Kim, H.S., Kim, H.S., Kim, K.S., Chang, K.T., Ryoo, Z.Y., Wang, S.M., Huh, T.L., and Lee, S.
Date: 2009
Source: Molecular genetics and genomics : MGG   281(1): 77-85 (Journal)
Registered Authors: Kim, Hyung-Seok
Keywords: Genome-wide analysis, SAGE, Mind bomb
MeSH Terms:
  • Animals
  • Base Sequence
  • DNA Primers/genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Genome-Wide Association Study
  • In Situ Hybridization
  • Mesoderm/embryology
  • Mutation
  • Neurogenesis/genetics
  • Oligonucleotide Array Sequence Analysis
  • Receptors, Notch/metabolism
  • Signal Transduction
  • Ubiquitin-Protein Ligases/genetics*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 19005681 Full text @ Mol. Genet. Genomics
Mind bomb (Mib) facilitates Notch signaling pathway by promoting the endocytosis of Notch ligand. The zebrafish mib ( ta52b ) mutant has a defect in its ubiquitin ligase activity which is necessary to inhibit the neurogenesis, resulting in a neuronal hyperplasia. Several genes regulated in the mib ( ta52b ) mutant have been well established, however, there were relatively few reports about the transcriptome profile. To identify the genes differentially expressed in the mib ( ta52b ) mutant, genome-wide analysis was performed using serial analysis of gene expression. Three hundred and thirty-five transcripts were identified whose expressions were significantly altered in the mib ( ta52b ) mutant as compared with the wild-type. Interestingly, it was suggested that the mib ( ta52b ) mutation may affect not only neurogenesis but also mesoderm development. These results provide new insights into Notch signaling pathway.