ZFIN ID: ZDB-PUB-081022-7
Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy
Keller, P.J., Schmidt, A.D., Wittbrodt, J., and Stelzer, E.H.
Date: 2008
Source: Science (New York, N.Y.) 322(5904): 1065-1069 (Journal)
Registered Authors: Wittbrodt, Jochen
Keywords: none
MeSH Terms:
  • Algorithms
  • Animals
  • Body Patterning
  • Cell Division*
  • Cell Nucleus/physiology
  • Databases, Factual
  • Embryo, Nonmammalian/cytology*
  • Embryonic Development*
  • Endoderm/embryology
  • Germ Layers/cytology
  • Germ Layers/embryology*
  • Germ Layers/physiology
  • Image Processing, Computer-Assisted
  • Mesoderm/embryology
  • Microscopy, Fluorescence/methods
  • Models, Biological
  • Motion Pictures
  • Mutation
  • Software
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • beta Catenin/analysis
PubMed: 18845710 Full text @ Science
ABSTRACT
A long-standing goal of biology is to map the behavior of all cells during vertebrate embryogenesis. We developed digital scanned laser light sheet fluorescence microscopy and recorded nuclei localization and movement in entire wild-type and mutant zebrafish embryos over the first 24 hours of development. Multiview in vivo imaging at 1.5 billion voxels per minute provides "digital embryos" (i.e., comprehensive databases of cell positions, divisions, and migratory tracks). Our analysis of global cell division patterns reveals a maternally defined initial morphodynamic symmetry break, which identifies the embryonic body axis. We further derive a model of germ layer formation and show that the mesendoderm forms from one-third of the embryo's cells in a single event. Our digital embryos, with 55 million nucleus entries, are provided as a resource.
ADDITIONAL INFORMATION