|ZFIN ID: ZDB-PUB-081022-20|
miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2
Pase, L., Layton, J.E., Kloosterman, W.P., Carradice, D., Waterhouse, P.M., and Lieschke, G.J.
|Source:||Blood 113(8): 1794-1804 (Journal)|
|Registered Authors:||Carradice, Duncan, Kloosterman, Wigard, Layton, Judy E., Lieschke, Graham J., Pase, Luke|
|PubMed:||18849488 Full text @ Blood|
Pase, L., Layton, J.E., Kloosterman, W.P., Carradice, D., Waterhouse, P.M., and Lieschke, G.J. (2009) miR-451 regulates zebrafish erythroid maturation in vivo via its target gata2. Blood. 113(8):1794-1804.
ABSTRACTWe demonstrate that in zebrafish the microRNA miR-451 plays a crucial role in promoting erythroid maturation, in part via its target transcript gata2. Zebrafish miR-144 and miR-451 are processed from a single precursor transcript selectively expressed in erythrocytes. In contrast to other hematopoietic mutants, the zebrafish mutant meunier (mnr) showed intact erythroid specification but diminished miR-144/451 expression. Although erythropoiesis initiated normally in mnr, erythrocyte maturation was morphologically retarded. Morpholino-knockdown of miR-451 increased erythrocyte immaturity in wild-type embryos, and miR-451 RNA duplexes partially rescued erythroid maturation in mnr, demonstrating a requirement and role for miR-451 in erythrocyte maturation. Mnr provided a selectively miR-144/451-deficient background facilitating studies to discern miRNA function and validate candidate targets. Amongst computer-predicted miR-451 targets potentially mediating these biological effects, the pro-stem cell transcription factor gata2 was an attractive candidate. In vivo reporter assays validated the predicted miR-451/gata2-3'UTR interaction, gata2 down-regulation was delayed in miR-451-knockdown and mnr embryos, and gata2 knockdown partially restored erythroid maturation in mnr, collectively confirming gata2 down-regulation as pivotal for miR-451-driven erythroid maturation. These studies define a new genetic pathway promoting erythroid maturation (mnr/miR-451/gata2) and provide a rare example of partial rescue of a mutant phenotype solely by miRNA overexpression.