PUBLICATION
Rhythmic patterns in phagocytosis and the production of reactive oxygen species by zebrafish leukocytes
- Authors
- Kaplan, J.E., Chrenek, R.D., Morash, J.G., Ruksznis, C.M., and Hannum, L.G.
- ID
- ZDB-PUB-080922-5
- Date
- 2008
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 151(4): 726-730 (Journal)
- Registered Authors
- Keywords
- circadian rhythm, clock, Danio rerio, immune, phagocytosis, respiratory burst, zebrafish
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Circadian Rhythm/immunology*
- Escherichia coli/immunology
- Female
- Kidney/cytology
- Kidney/immunology
- Kidney/metabolism
- Kinetics
- Leukocytes/immunology
- Leukocytes/metabolism
- Male
- Phagocytosis
- Reactive Oxygen Species/metabolism
- Respiratory Burst
- Staphylococcus aureus/immunology
- Zebrafish/immunology*
- Zebrafish/metabolism
- PubMed
- 18793741 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Kaplan, J.E., Chrenek, R.D., Morash, J.G., Ruksznis, C.M., and Hannum, L.G. (2008) Rhythmic patterns in phagocytosis and the production of reactive oxygen species by zebrafish leukocytes. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 151(4):726-730.
Abstract
Multiple components of vertebrate immune systems have been shown to exhibit circadian fluctuations. While the zebrafish is currently generating a wealth of information on the molecular pacemakers that may control circadian rhythms, there have been no reports of rhythmic activity in zebrafish leukocytes. In this study, we found that phagocytosis and the production of reactive oxygen species by zebrafish leukocytes varied significantly throughout twenty-four hour periods. A distinct peak in cellular ROS levels occurred before dawn, while the kinetics of respiratory burst responses were least rapid at this time of day. Phagocytosis of E. coli peaked late in the day, whereas there was no daily variation in phagocytosis of S. aureus. As seen in other species, the number of bacteria ingested per cell peaked during the night. These data provide direct evidence of rhythmic immune system activity, and demonstrate that zebrafish can be a valuable model in which to study the relationships between circadian gene expression, systemic pacemakers, and the activity of vertebrate immune system cells.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping