ZFIN ID: ZDB-PUB-080922-22
Novel caspase-suicide proteins for tamoxifen-inducible apoptosis
Chu, Y., Senghaas, N., Köster, R.W., Wurst, W., and Kühn, R.
Date: 2008
Source: Genesis (New York, N.Y. : 2000)   46(10): 530-536 (Journal)
Registered Authors: Köster, Reinhard W.
Keywords: caspase, apoptosis, tamoxifen, cell ablation, estrogen receptor
MeSH Terms:
  • Animals
  • Antineoplastic Agents, Hormonal/pharmacology*
  • Apoptosis/drug effects*
  • Apoptosis/genetics
  • Binding Sites/drug effects
  • Binding Sites/genetics
  • Caspases/genetics
  • Caspases/physiology*
  • Cell Line
  • Cell Line, Transformed
  • Genes, Transgenic, Suicide*/drug effects
  • HeLa Cells
  • Humans
  • Mice
  • Selective Estrogen Receptor Modulators/pharmacology
  • Tamoxifen/pharmacology*
  • Zebrafish
PubMed: 18802959 Full text @ Genesis
Taking advantage of a mutant estrogen receptor ligand binding domain (ER(T2)), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase-ER(T2) fusion proteins become specifically activated by the synthetic ligand 4-OH- tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms.