PUBLICATION
Fgf signaling in the zebrafish adult brain: Association of Fgf activity with ventricular zones but not cell proliferation
- Authors
- Topp, S., Stigloher, C., Komisarczuk, A.Z., Adolf, B., Becker, T.S., and Bally-Cuif, L.
- ID
- ZDB-PUB-080801-17
- Date
- 2008
- Source
- The Journal of comparative neurology 510(4): 422-439 (Journal)
- Registered Authors
- Adolf, Birgit, Bally-Cuif, Laure, Becker, Thomas S., Komisarczuk, Anna Zofia, Stigloher, Christian, Topp, Stefanie
- Keywords
- Fgf, P-ERK, sprouty, zebrafish, neural progenitor, adult brain
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Brain/physiology*
- Cell Division
- Cerebral Ventricles/cytology*
- Cerebral Ventricles/physiology*
- Fibroblast Growth Factors/genetics*
- Fibroblast Growth Factors/physiology
- Genes, Reporter
- Glial Fibrillary Acidic Protein/genetics
- Green Fluorescent Proteins/genetics
- Hypothalamus/growth & development
- Hypothalamus/physiology
- Immunohistochemistry
- In Situ Hybridization
- Mesencephalon/physiology
- Receptors, Fibroblast Growth Factor/genetics
- Rhombencephalon/physiology
- Signal Transduction
- Zebrafish
- PubMed
- 18666124 Full text @ J. Comp. Neurol.
Citation
Topp, S., Stigloher, C., Komisarczuk, A.Z., Adolf, B., Becker, T.S., and Bally-Cuif, L. (2008) Fgf signaling in the zebrafish adult brain: Association of Fgf activity with ventricular zones but not cell proliferation. The Journal of comparative neurology. 510(4):422-439.
Abstract
The zebrafish adult brain contains numerous neural progenitors and is a good model to approach the general mechanisms of adult neural stem cell maintenance and neurogenesis. Here we use this model to test for a correlation between Fgf signaling and cell proliferation in adult progenitor zones. We report expression of Fgf signals (fgf3,4,8a,8b,17b), receptors (fgfr1-4), and targets (erm, pea3, dusp6, spry1,2,4, and P-ERK) and document that genes of the embryonic fgf8 synexpression group acquire strikingly divergent patterns in the adult brain. We further document the specific expression of fgf3, fgfr1-3, dusp6, and P-ERK in ventricular zones, which contain neural progenitors. In these locations, however, a comparison at the single-cell level of fgfr/P-ERK expression with bromo-deoxy-uridine (BrdU) incorporation and the proliferation marker MCM5 indicates that Fgf signaling is not specifically associated with proliferating progenitors. Rather, it correlates with the ventricular radial glia state, some of which only are progenitors. Together these results stress the importance of Fgf signaling in the adult brain and establish the basis to study its function in zebrafish, in particular in relation to adult neurogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping