ZFIN ID: ZDB-PUB-080622-8
Zinc finger-based knockout punches for zebrafish genes
Ekker, S.C.
Date: 2008
Source: Zebrafish 5(2): 121-123 (Review)
Registered Authors: Ekker, Stephen C.
Keywords: none
MeSH Terms: Animals; Deoxyribonucleases/genetics; Deoxyribonucleases/metabolism; Gene Deletion*; Gene Expression Regulation/physiology (all 7) expand
PubMed: 18554175 Full text @ Zebrafish
The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental organism for understanding human disease. This ZFN-based technology to generate targeted knockouts in this aquatic animal opens the door to an array of new biological models of human disease and genetic testing.