PUBLICATION
Zinc finger-based knockout punches for zebrafish genes
- Authors
- Ekker, S.C.
- ID
- ZDB-PUB-080622-8
- Date
- 2008
- Source
- Zebrafish 5(2): 121-123 (Review)
- Registered Authors
- Ekker, Stephen C.
- Keywords
- none
- MeSH Terms
-
- Zebrafish/genetics*
- Animals
- Zinc Fingers*
- Gene Expression Regulation/physiology
- Deoxyribonucleases/genetics
- Deoxyribonucleases/metabolism
- Gene Deletion*
- PubMed
- 18554175 Full text @ Zebrafish
Citation
Ekker, S.C. (2008) Zinc finger-based knockout punches for zebrafish genes. Zebrafish. 5(2):121-123.
Abstract
The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental organism for understanding human disease. This ZFN-based technology to generate targeted knockouts in this aquatic animal opens the door to an array of new biological models of human disease and genetic testing.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping