PUBLICATION
Movement disorder and neuromuscular change in zebrafish embryos after exposure to caffeine
- Authors
- Chen, Y.H., Huang, Y.H., Wen, C.C., Wang, Y.H., Chen, W.L., Chen, L.C., and Tsay, H.J.
- ID
- ZDB-PUB-080602-11
- Date
- 2008
- Source
- Neurotoxicology and teratology 30(5): 440-447 (Journal)
- Registered Authors
- Chen, Yau-Hung, Tsay, Huey-Jen
- Keywords
- Acetylcholine receptor, Caffeine, Embryonic development, Motor neuron, Muscle, Zebrafish
- MeSH Terms
-
- Embryo, Nonmammalian/abnormalities
- Embryo, Nonmammalian/drug effects*
- Sensation Disorders/chemically induced
- Sensation Disorders/pathology
- Sensation Disorders/physiopathology
- Neuromuscular Junction/abnormalities
- Neuromuscular Junction/drug effects
- Neuromuscular Junction/physiopathology
- Embryonic Development/drug effects
- Embryonic Development/physiology
- Toxicity Tests
- Antibodies, Monoclonal
- Caffeine/toxicity*
- Muscle Fibers, Skeletal/drug effects
- Muscle Fibers, Skeletal/pathology
- Animals
- Zebrafish
- Phosphodiesterase Inhibitors/toxicity
- Time Factors
- Dose-Response Relationship, Drug
- Survival Rate
- Spinal Cord/abnormalities
- Spinal Cord/drug effects*
- Muscle, Skeletal/abnormalities
- Muscle, Skeletal/drug effects*
- Muscle, Skeletal/innervation
- Dyskinesia, Drug-Induced/pathology
- Dyskinesia, Drug-Induced/physiopathology*
- Disease Models, Animal
- Motor Neurons/drug effects*
- Motor Neurons/pathology
- PubMed
- 18508234 Full text @ Neurotoxicol. Teratol.
Citation
Chen, Y.H., Huang, Y.H., Wen, C.C., Wang, Y.H., Chen, W.L., Chen, L.C., and Tsay, H.J. (2008) Movement disorder and neuromuscular change in zebrafish embryos after exposure to caffeine. Neurotoxicology and teratology. 30(5):440-447.
Abstract
Though caffeine is broadly distributed in many plants and foods, little is known about the teratogenic effects of caffeine during early embryonic development. Here, we used zebrafish as a model to test toxicity and teratogenicity since they have transparent eggs, making the organogenesis of zebrafish embryos easier to observe. When the exposure doses of caffeine were less than 150 ppm (17.5, 35, 50, 100 and 150 ppm), the zebrafish embryos exhibited no significant differences in survival rates after comparison with vehicle-control (0 ppm) group. As the exposure dosages increased, the survival rates decreased. No embryos survived after treatment with 300 ppm caffeine or higher dosages. The most evident change in embryos treated with caffeine was a shorter body length (vehicle-control: 3.26+/-0.01 mm, n=49; vs 150 ppm of caffeine: 2.67+/-0.03 mm, n=50). In addition, caffeine-treated embryos exhibited significantly reduced tactile sensitivity frequencies of touch-induced movement (vehicle-control: 9.93+/-0.77 vs 17.5-150 ppm caffeine: 5.37+/-0.52-0.10+/-0.06). Subtle changes are easily observed by staining with specific monoclonal antibodies F59, Znp1 and Zn5 to detect morphological changes in muscle fibers, primary motor axons and secondary motor axon projections, respectively. Our data show that the treatment of caffeine leads to misalignment of muscle fibers and motor neuron defects, especially secondary motor neuron axonal growth defects.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping