PUBLICATION
Characterization of Kras-mediated pancreatic tumorigenesis in zebrafish
- Authors
- Davison, J.M., Woo Park, S., Rhee, J.M., and Leach, S.D.
- ID
- ZDB-PUB-080421-5
- Date
- 2008
- Source
- Methods in enzymology 438: 391-417 (Journal)
- Registered Authors
- Leach, Steven D.
- Keywords
- none
- MeSH Terms
-
- ras Proteins/adverse effects*
- ras Proteins/genetics*
- Chromosomes, Artificial, Bacterial
- Green Fluorescent Proteins/genetics
- Zebrafish
- Gene Expression Regulation, Neoplastic
- In Situ Hybridization, Fluorescence
- Cell Differentiation/drug effects
- Pancreatic Neoplasms/etiology*
- Pancreatic Neoplasms/metabolism
- Pancreatic Neoplasms/pathology
- Immunohistochemistry
- RNA, Antisense/metabolism
- Animals
- Humans
- Genes, ras/physiology*
- Disease Models, Animal
- Pancreas, Exocrine/embryology
- Pancreas, Exocrine/metabolism
- Animals, Genetically Modified
- PubMed
- 18413263 Full text @ Methods Enzymol.
Citation
Davison, J.M., Woo Park, S., Rhee, J.M., and Leach, S.D. (2008) Characterization of Kras-mediated pancreatic tumorigenesis in zebrafish. Methods in enzymology. 438:391-417.
Abstract
Activating Kras mutations are a pervasive and characteristic feature of human pancreatic cancer. In order to examine the earliest in vivo effects of oncogenic Kras expression in the exocrine pancreas, we generated two lines of zebrafish expressing eGFP alone or eGFP fused to human Kras with an activating mutation in codon 12 (Kras(G12V)) driven by ptf1a regulatory elements using a BAC recombineering strategy (Park et al., 2008). In this review, we describe the techniques that we used to observe the effects of eGFP-Kras(G12V) expression in pancreatic progenitor cells of the zebrafish embryo, as well as techniques used to characterize malignant pancreatic tumors in the adult zebrafish. This zebrafish model of pancreatic neoplasia provides a unique view of the effects of oncogenic Kras in the embryonic pancreas and suggests that the zebrafish will be a useful model organism in which to study the biology of Kras-initiated pancreatic neoplasia.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping