PUBLICATION
The Signal Recognition Particle Pathway is Required for Virulence in Streptococcus pyogenes
- Authors
- Rosch, J.W., Vega, L.A., Beyer, J., Lin, A., and Caparon, M.G.
- ID
- ZDB-PUB-080421-4
- Date
- 2008
- Source
- Infection and Immunity 76(6): 2612-2619 (Journal)
- Registered Authors
- Caparon, Michael
- Keywords
- none
- MeSH Terms
-
- Animals
- Computational Biology
- Female
- Gene Deletion
- Gene Expression Regulation/physiology
- Mice
- Myositis/microbiology
- Necrosis/microbiology
- Signal Recognition Particle/genetics
- Signal Recognition Particle/metabolism*
- Streptococcal Infections/microbiology*
- Streptococcus pyogenes/metabolism*
- Streptococcus pyogenes/pathogenicity*
- Virulence
- Zebrafish
- PubMed
- 18411293 Full text @ Infect. Immun.
Citation
Rosch, J.W., Vega, L.A., Beyer, J., Lin, A., and Caparon, M.G. (2008) The Signal Recognition Particle Pathway is Required for Virulence in Streptococcus pyogenes. Infection and Immunity. 76(6):2612-2619.
Abstract
The signal recognition particle (SRP) pathway is a universally conserved pathway for targeting polypeptides for secretion via the co-translational pathway. In particular, the SRP pathway is thought to be the main mechanism for targeting polypeptides in Gram-positive bacteria, including a number of important human pathogens. Though widely considered to be an essential cellular component, recent advances have indicated this pathway may be dispensable in Gram-positive bacteria of the genus Streptococcus under in vitro conditions. However, its importance for the pathogenesis of streptococcal disease is unknown. In this study, we investigated the importance of the SRP pathway for virulence factor secretion in the human pathogen Streptococcus pyogenes. While not essential for viability in vitro, SRP mutants demonstrated a medium-specific growth defect that could be rescued by the addition of glucose. We also observed that a distinct subset of virulence factors were dependent upon the SRP pathway for secretion, whereas others were completely independent of this pathway. Significantly, deletion of the SRP pathway resulted in mutants that were highly attenuated in both a zebrafish model of necrotic myositis and a murine subcutaneous ulcer model, highlighting the importance of this pathway in vivo. These studies emphasize the importance of the SRP pathway for the in vivo survival and pathogenesis of S. pyogenes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping