PUBLICATION
Gender-specific proteomic responses in zebrafish liver following exposure to a selected mixture of brominated flame retardants
- Authors
- Kling, P., Norman, A., Andersson, P.L., Norrgren, L., and Förlin, L.
- ID
- ZDB-PUB-080227-12
- Date
- 2008
- Source
- Ecotoxicology and environmental safety 71(2): 319-327 (Journal)
- Registered Authors
- Norrgren, Leif
- Keywords
- Fish, Proteomics, BFR, MALDI-TOF
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Female
- Flame Retardants/toxicity*
- Gene Expression Profiling
- Gene Expression Regulation/drug effects
- Hydrocarbons, Brominated/toxicity*
- Liver/drug effects*
- Liver/metabolism*
- Male
- Proteomics
- Sex Characteristics
- Zebrafish/metabolism*
- Zebrafish Proteins/biosynthesis
- PubMed
- 18258299 Full text @ Ecotoxicol. Environ. Saf.
- CTD
- 18258299
Citation
Kling, P., Norman, A., Andersson, P.L., Norrgren, L., and Förlin, L. (2008) Gender-specific proteomic responses in zebrafish liver following exposure to a selected mixture of brominated flame retardants. Ecotoxicology and environmental safety. 71(2):319-327.
Abstract
Proteomic effect screening in zebrafish liver was performed to generate hypotheses following exposure (21 days) to a structurally diverse mixture of brominated flame retardants (BFRs). Fish were exposed to two doses (10 and 100nmol/g feed). Two-dimensional gel-electrophoresis, image analysis and MALDI-TOF mass-spectrometry revealed 13 and 19 significant responses in males and females, respectively. Effects on proteins related to cellular maintenance and stress were observed in both genders. Regulated proteins were gender-specific, but functionally indicated common protective responses (peroxiredoxin 6 and Zgc:92891 in males and transketolase in females) suggesting oxidative stress. Betaine homocysteine methyltransferase (BHMT) was induced in both genders. In addition a female-specific downregulation of ironhomeostatic proteins (iron-regulatory protein 1 and transferrin) were observed. Our proteomic approach revealed novel responses that suggest important gender-specific sensitiviy to BFRs that should be considered when interpreting adverse effects of BFRs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping