PUBLICATION
siRNA specific to Pdx-1 disturbed the formation of the islet in early zebrafish embryos
- Authors
- Chen, S., Huang, J., Yuan, G., Chen, Q., Huang, N., and Xie, F.
- ID
- ZDB-PUB-080226-15
- Date
- 2007
- Source
- Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 27(6): 639-642 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian*
- Homeodomain Proteins/genetics
- Homeodomain Proteins/metabolism*
- Islets of Langerhans/cytology
- Islets of Langerhans/embryology*
- Islets of Langerhans/metabolism
- RNA Interference
- RNA, Small Interfering/genetics*
- Trans-Activators/genetics
- Trans-Activators/metabolism*
- Zebrafish
- PubMed
- 18231730 Full text @ J. Huazhong Univ. Sci. Technolog. Med. Sci.
Citation
Chen, S., Huang, J., Yuan, G., Chen, Q., Huang, N., and Xie, F. (2007) siRNA specific to Pdx-1 disturbed the formation of the islet in early zebrafish embryos. Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban. 27(6):639-642.
Abstract
Pdx-1, an important transcription factor highlighting in the early pancreatic development, islet functions and pancreatic disorders, needs to be more investigated in zebrafish, and siRNA is still seldom applied in zebrafish embryo-related research. Our aim was to explore the role of pdx-1 in pancreatic development of zebrafish embryos by using siRNA approach. Microinjection, reverse transcriptase-PCR (RT-PCR), in situ hybridization and immunofluorescent staining were used in this research, and the morphology of the islet in normal zebrafish embryos, and in those treated with the siRNA specific to pdx-1 (siPDX-1) or siGFP was observed and compared. The expression of pdx-1 was detected in the stages of 1-cell, 2-cell, 4-cell, 8-cell, 16-cell, 16-hour by RT-PCT. The in situ hybridization and immunofluorescent staining results showed that siPDX-1 disturbed the formation of the islet in zebrafish embryos. Pdx-1 played multiple roles in maintaining the phenotype of the islet during embryogenesis in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping