PUBLICATION

CNS-derived glia ensheath peripheral nerves and mediate motor root development

Authors
Kucenas, S., Takada, N., Park, H.C., Woodruff, E., Broadie, K., and Appel, B.
ID
ZDB-PUB-080124-9
Date
2008
Source
Nature Neuroscience   11(2): 143-151 (Journal)
Registered Authors
Appel, Bruce, Kucenas, Sarah, Park, Hae-Chul, Takada, Norio
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Axons/physiology*
  • Cell Differentiation
  • Central Nervous System/cytology*
  • Embryo, Nonmammalian
  • Fishes
  • Gene Expression Regulation, Developmental/physiology
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Microscopy, Electron, Transmission
  • Morpholines/pharmacology
  • Motor Neurons/physiology*
  • Myelin Sheath/physiology*
  • Myelin Sheath/ultrastructure
  • Neuroglia/physiology*
  • Neuroglia/ultrastructure
  • Peripheral Nerves/cytology
  • Peripheral Nerves/physiology*
  • Phosphoproteins/genetics
  • Phosphoproteins/metabolism
  • Schwann Cells/physiology
  • Schwann Cells/ultrastructure
  • Spinal Cord/cytology
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Veratrum Alkaloids/pharmacology
  • Zebrafish Proteins/genetics
  • Zonula Occludens-1 Protein
PubMed
18176560 Full text @ Nat. Neurosci.
Abstract
Motor function requires that motor axons extend from the spinal cord at regular intervals and that they are myelinated by Schwann cells. Little attention has been given to another cellular structure, the perineurium, which ensheaths the motor nerve, forming a flexible, protective barrier. Consequently, the origin of perineurial cells and their roles in motor nerve formation are poorly understood. Using time-lapse imaging in zebrafish, we show that perineurial cells are born in the CNS, arising as ventral spinal-cord glia before migrating into the periphery. In embryos lacking perineurial glia, motor neurons inappropriately migrated outside of the spinal cord and had aberrant axonal projections, indicating that perineurial glia carry out barrier and guidance functions at motor axon exit points. Additionally, reciprocal signaling between perineurial glia and Schwann cells was necessary for motor nerve ensheathment by both cell types. These insights reveal a new class of CNS-born glia that critically contributes to motor nerve development.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes