PUBLICATION
Discovery of a gonad-specific IGF subtype in teleost
- Authors
- Wang, D., Jiao, B., Hu, C., Huang, X., Liu, Z., and Cheng, C.H.
- ID
- ZDB-PUB-080102-14
- Date
- 2008
- Source
- Biochemical and Biophysical Research Communications 367(2): 336-341 (Journal)
- Registered Authors
- Keywords
- cDNA cloning, Gene expression, Novel IGF, Teleost
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Gonads/metabolism*
- Molecular Sequence Data
- Sexual Development/physiology*
- Somatomedins/chemistry*
- Somatomedins/genetics
- Somatomedins/metabolism*
- Species Specificity
- Tilapia/metabolism*
- Tissue Distribution
- Zebrafish/metabolism*
- PubMed
- 18166148 Full text @ Biochem. Biophys. Res. Commun.
Citation
Wang, D., Jiao, B., Hu, C., Huang, X., Liu, Z., and Cheng, C.H. (2008) Discovery of a gonad-specific IGF subtype in teleost. Biochemical and Biophysical Research Communications. 367(2):336-341.
Abstract
Distinct from the conventional IGFs (IGF-1 and IGF-2), here we report the identification of a novel IGF (herein called IGF-3) encoded by a separate gene from teleost species. The IGF-3 cDNA sequences were cloned from tilapia and zebrafish, and predicted from the medaka genome and EST databases. Similar to IGF-1 and IGF-2, IGF-3 is also comprised of five domains (B, C, A, D, E) with a similar tertiary protein structure, despite a low sequence homology among them. Phylogenetic analysis reveals that the IGF-3 sequences from different teleosts cluster to form a distinctive clade separate from IGF-1 and IGF-2. The expression of this novel IGF-3 is gonad-specific and starts early in fish development. In situ hybridization revealed that IGF-3 is expressed in the somatic cells and later in granulosa cells of the ovary, and in the interstitial cells of the testis. These findings highlight the importance of this novel IGF in teleost gonadal development and reproduction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping