ZFIN ID: ZDB-PUB-071125-23
OFF ganglion cells cannot drive the optokinetic reflex in zebrafish
Emran, F., Rihel, J., Adolph, A.R., Wong, K.Y., Kraves, S., and Dowling, J.E.
Date: 2007
Source: Proceedings of the National Academy of Sciences of the United States of America   104(48): 19126-19131 (Journal)
Registered Authors: Dowling, John E., Emran, Farida, Kraves, Sebastian, Rihel, Jason, Wong, Kwoon
Keywords: extracellular recordings, ON and OFF retinal pathway, optokinetic response, retina, retinal ganglion cells
MeSH Terms:
  • Action Potentials/radiation effects
  • Aminobutyrates/pharmacology
  • Animals
  • Aspartic Acid/pharmacology
  • Electroretinography
  • Motion Perception/physiology
  • Nystagmus, Optokinetic/physiology*
  • Phosphoric Monoester Hydrolases/deficiency
  • Phosphoric Monoester Hydrolases/genetics
  • Photic Stimulation
  • Photoreceptor Cells/abnormalities
  • Retinal Bipolar Cells/physiology
  • Retinal Ganglion Cells/drug effects
  • Retinal Ganglion Cells/physiology*
  • Vision Disorders/genetics
  • Visual Pathways/drug effects
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Zebrafish Proteins/deficiency
  • Zebrafish Proteins/genetics
PubMed: 18025459 Full text @ Proc. Natl. Acad. Sci. USA
Whereas the zebrafish retina has long been an important model system for developmental and genetic studies, little is known about the responses of the inner retinal neurons. Here we report single-unit ganglion cell recordings from 5- to 6-day-old zebrafish larvae. In wild-type larvae we identify at least five subtypes of ganglion cell responses to full-field illumination, with ON-OFF and ON-type cells predominating. In the nrc mutant retina, in which the photoreceptor terminals develop abnormally, we observe normal OFF responses but abnormal ON-OFF responses and no ON responses. Previously characterized as blind, these mutants lack an optokinetic reflex (OKR), but in another behavioral assay nrc mutant fish have near-normal responses to the offset of light and slow and sluggish responses to the onset of light . Pharmacological block of the ON pathway mimics most of the nrc visual defects. We conclude that the abnormal photoreceptor terminals in nrc mutants predominantly perturb the ON pathway and that the ON pathway is necessary to drive the OKR in larval zebrafish.