ZFIN ID: ZDB-PUB-071029-12
Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo
Yang, L., Kemadjou, J.R., Zinsmeister, C., Legradi, J., Bauer, M., Muller, F., Pankratz, M., Jakel, J., and Straehle, U.
Date: 2007
Source: Genome biology   8(10): R227 (Journal)
Registered Authors: Kemadjou Njiwa, Jules (Richard), Legradi, Jessica, Müller, Ferenc, Strähle, Uwe, Yang, Lixin
Keywords: none
Microarrays: GEO:GSE9357
MeSH Terms:
  • Animals
  • DNA, Complementary/genetics
  • Embryo, Nonmammalian/drug effects
  • Gene Expression/drug effects*
  • Gene Expression Profiling*
  • Noxae/toxicity*
  • Oligonucleotide Array Sequence Analysis
  • Toxicogenetics/methods*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed: 17961207 Full text @ Genome Biol.
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ABSTRACT
BACKGROUND: Early life stages are generally most sensitive to toxic effects. Our knowledge on the action of man-made chemicals on the developing vertebrate embryo is, however, rather limited. We addressed here in a systematic manner the toxicogenomic response of the zebrafish embryo by asking whether distinct chemicals would induce specific transcriptional profiles. RESULTS: We exposed zebrafish embryos to a range of environmental toxicants and measured the changes in gene expression profiles by hybridizing cDNA to an oligonucleotide microarray. Several hundred genes responded significantly to at least one of the 11 toxicants tested. We obtained specific expression profiles for each of the chemicals and could predict the identity of the toxicant from the expression profiles with high probability. Changes in gene expression were observed at toxicant concentrations that did not cause morphological effects. The toxicogenomic profiles were highly stage-specific and we detected tissue specific-gene responses underscoring the sensitivity of the assay system. CONCLUSION: Our results demonstrate that the genome of the zebrafish embryo responds to toxicant exposure in a highly sensitive and specific manner. Our work provides proof-of-principle for the use of the zebrafish embryo as a toxicogenomic model and highlights its potential for systematic, large-scale analysis of the effects of chemicals on the developing vertebrate embryo.
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