PUBLICATION
The teleost intervertebral region acts as a growth center of the centrum: In vivo visualization of osteoblasts and their progenitors in transgenic fish
- Authors
- Inohaya, K., Takano, Y., and Kudo, A.
- ID
- ZDB-PUB-071009-5
- Date
- 2007
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 236(11): 3031-3046 (Journal)
- Registered Authors
- Inohaya, Keiji
- Keywords
- bone, vertebral column, osteoblast, teleost, transgenic
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Bone Development*
- Bone and Bones
- Cell Differentiation*
- Fishes
- Green Fluorescent Proteins/metabolism
- Oryzias/embryology*
- Oryzias/metabolism
- Osteoblasts/cytology*
- Osteoblasts/metabolism
- Osteocalcin/metabolism
- Spine/anatomy & histology
- Spine/embryology*
- Twist-Related Protein 1/metabolism
- Zebrafish/embryology*
- PubMed
- 17907202 Full text @ Dev. Dyn.
Citation
Inohaya, K., Takano, Y., and Kudo, A. (2007) The teleost intervertebral region acts as a growth center of the centrum: In vivo visualization of osteoblasts and their progenitors in transgenic fish. Developmental Dynamics : an official publication of the American Association of Anatomists. 236(11):3031-3046.
Abstract
The vertebral column is a defined feature of vertebrates. In birds and mammals, the sclerotome yields cartilaginous material for the vertebral column. In teleosts, however, it remains uncertain whether the sclerotome participates in vertebral column formation. To investigate osteoblast development in the teleost, we established transgenic systems that allow in vivo observation of osteoblasts and their progenitors marked by fluorescence of DsRed and enhanced green fluorescent protein (EGFP), respectively. In twist-EGFP transgenic medaka, EGFP-positive cells first appeared in the ventromedial portion of respective somites corresponding to the sclerotome, migrated dorsally around the notochord, and concentrated in the intervertebral regions. Ultrastructural analysis of the intervertebral regions revealed that some of these cells were directly located on the osteoidal surface of the perichordal centrum, and enriched with rough endoplasmic reticulum in their cytoplasm. By using the double transgenic medaka of twist-EGFP and osteocalcin-DsRed, we clarified that the EGFP-positive cells in the intervertebral region differentiated into mature osteoblasts expressing the DsRed. In vivo bone labeling in fact confirmed active matrix formation and mineralization of the perichordal centrum exclusively in the intervertebral region of zebrafish larvae as well as medaka larvae. These findings strongly suggest that the teleost intervertebral region acts as a growth center of the perichordal centrum, where the sclerotome-derived cells differentiate into osteoblasts.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping