PUBLICATION
Expression of an intron-containing beta-tubulin mRNA in catfish olfactory epithelium
- Authors
- Bettini, E., and Margolis, F.L.
- ID
- ZDB-PUB-070920-3
- Date
- 2007
- Source
- Journal of Molecular Histology 38(6): 571-579 (Journal)
- Registered Authors
- Keywords
- PCR, β-Tubulin, Ictalurus punctatus, In situ hybridization, Olfactory
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Northern
- DNA, Complementary/metabolism
- Gene Expression
- Ictaluridae/genetics*
- Ictaluridae/metabolism
- In Situ Hybridization
- Introns*
- Molecular Sequence Data
- Olfactory Mucosa/cytology
- Olfactory Mucosa/metabolism*
- Olfactory Receptor Neurons/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism*
- Sequence Alignment
- Tubulin/chemistry
- Tubulin/genetics*
- Tubulin/metabolism
- PubMed
- 17849223 Full text @ J. Mol. Histol.
Citation
Bettini, E., and Margolis, F.L. (2007) Expression of an intron-containing beta-tubulin mRNA in catfish olfactory epithelium. Journal of Molecular Histology. 38(6):571-579.
Abstract
beta-Tubulin genes code for very similar proteins, sharing extensive identity in amino acid sequence within and across species, each of which manifests characteristic patterns of cell and tissue expression. While searching for olfactory specific mRNAs in the channel catfish (Ictalurus punctatus), we isolated a novel beta-tubulin cDNA. In the putative ORF, 1298 nucleotides were 80-88% identical to cloned cDNAs from zebrafish to human for beta-tubulin isotype IVb. This ORF is interrupted by an insert of 111 nucleotides located between the regions corresponding to exons 2 and 3 in other species. This insert lacks similarity to any sequence in the NCBI databases. We showed that this novel cDNA fragment hybridizes specifically to catfish olfactory epithelium mRNA on Northern analysis. Here we demonstrate by in situ analysis of catfish olfactory epithelium that the expression of this mRNA is spatially restricted to the outer two-thirds of each olfactory lamella where olfactory receptor neurons reside. These results suggest that this nucleotide sequence is the result of incomplete RNA transcript processing. The growing awareness of the regulatory roles played by RNAs transcribed from intronic regions of genes suggests that this observation may have relevance to regulation of gene expression in olfactory tissue during development and axon targeting.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping