|ZFIN ID: ZDB-PUB-070907-30|
Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)
Huisken, J., and Stainier, D.Y.
|Source:||Optics letters 32(17): 2608-2610 (Journal)|
|Registered Authors:||Stainier, Didier|
|PubMed:||17767321 Full text @ Opt. Lett.|
Huisken, J., and Stainier, D.Y. (2007) Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM). Optics letters. 32(17):2608-2610.
ABSTRACTMultidirectional selective plane illumination microscopy (mSPIM) reduces absorption and scattering artifacts and provides an evenly illuminated focal plane. mSPIM solves two common problems in light-sheet-based imaging techniques: The shadowing in the excitation path due to absorption in the specimen is eliminated by pivoting the light sheet; the spread of the light sheet by scattering in the sample is compensated by illuminating the sample consecutively from opposing directions. The resulting two images are computationally fused yielding a superior image. The effective light sheet is thinner, and the axial resolution is increased by square root 2 over single-directional SPIM. The multidirectional illumination proves essential in biological specimens such as millimeter-sized embryos. The performance of mSPIM is demonstrated by the imaging of live zebrafish embryos.