Light-controlled gene silencing in zebrafish embryos
- Shestopalov, I.A., Sinha, S., and Chen, J.K.
- Nature Chemical Biology 3(10): 650-651 (Journal)
- Registered Authors
- Chen, James K., Sinha, Surajit
- MeSH Terms
- Body Patterning
- Embryo, Nonmammalian/physiology
- Embryo, Nonmammalian/radiation effects*
- Gene Expression Regulation, Developmental/physiology
- Gene Expression Regulation, Developmental/radiation effects*
- Gene Silencing/physiology
- Gene Silencing/radiation effects*
- Peptide Chain Initiation, Translational
- RNA Splicing/drug effects*
- RNA Splicing/physiology
- Ultraviolet Rays
- 17717538 Full text @ Nat. Chem. Biol.
Shestopalov, I.A., Sinha, S., and Chen, J.K. (2007) Light-controlled gene silencing in zebrafish embryos. Nature Chemical Biology. 3(10):650-651.
Functional genomic studies in zebrafish frequently use synthetic oligonucleotides called morpholinos that block RNA splicing or translation. However, the constitutive activity of these reagents limits their experimental utility. We report here the synthesis of a photoactivatable morpholino targeting the no tail (ntl) gene. This caged reagent permits spatiotemporal gene regulation in vivo and the photochemical generation of functionally mosaic organisms.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes