PUBLICATION
Mouse lycat controls the development of hematopoietic and endothelial lineages during in vitro ES cell differentiation
- Authors
- Wang, C., Faloon, P.W., Tan, Z., Lv, Y., Zhang, P., Ge, Y., Deng, H., and Xiong, J.W.
- ID
- ZDB-PUB-070813-32
- Date
- 2007
- Source
- Blood 110(10): 3601-3609 (Journal)
- Registered Authors
- Faloon, Patrick, Xiong, Jing-Wei
- Keywords
- none
- MeSH Terms
-
- Endothelial Cells/cytology*
- Zebrafish/genetics
- Bone Marrow Cells/cytology
- Hematopoiesis/genetics*
- Acyltransferases/genetics
- Acyltransferases/physiology*
- Cardiolipins/chemistry
- Cardiolipins/metabolism
- Embryonic Stem Cells/cytology*
- Cell Lineage/genetics*
- Cells, Cultured
- Gene Expression/drug effects
- Cell Differentiation/genetics*
- Organisms, Genetically Modified
- Transfection
- Gene Expression Profiling
- RNA, Small Interfering/pharmacology
- Mice
- Animals
- Oligonucleotide Array Sequence Analysis
- PubMed
- 17675553 Full text @ Blood
Citation
Wang, C., Faloon, P.W., Tan, Z., Lv, Y., Zhang, P., Ge, Y., Deng, H., and Xiong, J.W. (2007) Mouse lycat controls the development of hematopoietic and endothelial lineages during in vitro ES cell differentiation. Blood. 110(10):3601-3609.
Abstract
The blast-colony-forming cell (BL-CFC) was identified as an equivalent to the hemangioblast during in vitro ES cell differentiation. However, it remains largely unknown what are the molecular mechanisms underlying the generation of the BL-CFC. Here we report the isolation of mouse lysocardiolipin acyltransferase (Lycat) based on homology to zebrafish lycat, a candidate gene for the cloche locus. Mouse Lycat is expressed in hematopoietic organs and is enriched in the Lin(-)C-Kit(+)Sca-1(+) hematopoietic stem cells in bone marrow and in the Flk1(+)/hCD4(+)(Scl(+)) hemangioblast population in embryoid bodies. The forced Lycat transgene leads to increased mRNA expression of hematopoietic and endothelial genes as well as increased blast colonies and their progenies, endothelial and hematopoietic lineages. The Lycat siRNA transgene leads to a decrease expression of hematopoietic and endothelial genes. An unbiased genome-wide microarray analysis further substantiates that the forced Lycat transgene specifically up-regulates a set of genes related to hemangioblasts, hematopoietic and endothelial lineages. Therefore, mouse Lycat plays an important role in the early specification of hematopoietic and endothelial cells, probably acting at the level of the hemangioblast.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping