ZFIN ID: ZDB-PUB-070806-9
Molecular changes associated with teratogen-induced cyclopia
Loucks, E.J., Schwend, T., and Ahlgren, S.C.
Date: 2007
Source: Birth Defects Research Part A: Clinical and Molecular Teratology 79(9): 642-651 (Journal)
Registered Authors: Ahlgren, Sara, Loucks, Evyn
Keywords: zebrafish, cyclopia, gli, six3b, dlx3b, telencephalon
MeSH Terms:
  • Animals
  • Base Sequence
  • Colforsin/toxicity
  • DNA Primers/genetics
  • Ethanol/toxicity
  • Eye Abnormalities/chemically induced*
  • Eye Abnormalities/embryology
  • Eye Abnormalities/genetics*
  • Eye Proteins/genetics
  • Gene Expression Regulation, Developmental/drug effects
  • Genes, Homeobox/drug effects
  • Goosecoid Protein/genetics
  • Hedgehog Proteins/genetics
  • Homeodomain Proteins/genetics
  • Kruppel-Like Transcription Factors/genetics
  • Nerve Tissue Proteins/genetics
  • Teratogens/toxicity*
  • Veratrum Alkaloids/toxicity
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics
PubMed: 17647295 Full text @ Birth Defects Res. A Clin. Mol. Teratol.
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ABSTRACT
BACKGROUND: Exposure of zebrafish embryos to a number of teratogens results in cyclopia, but little is known about the underlying molecular changes. METHODS: Using zebrafish embryos, we compare the effects cyclopamine, forskolin, and ethanol delivered starting just before gastrulation, on gene expression in early axial tissues and forebrain development. RESULTS: Although all three teratogens suppress gli1 expression, they do so with variable kinetics, suggesting that while suppression of Shh signaling is a common outcome of these three teratogens, it is not a common cause of the cyclopia. Instead, all teratogens studied produce a series of changes in the expression of gsc and six3b present in early axial development, as well as a later suppression of neural crest cell marker dlx3b. Ethanol and forskolin, but not cyclopamine, exposure reduced anterior markers, which most likely contributes to the cyclopic phenotype. CONCLUSIONS: These data suggest that each teratogen exposure leads to a unique set of molecular changes that underlie the single phenotype of cyclopia.
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