ZFIN ID: ZDB-PUB-070629-29
The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis
Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U.
Date: 2007
Source: Developmental Biology   308(1): 133-143 (Journal)
Registered Authors: Behra, Martine, Etard, Christelle, Fischer, Nadine, Geisler, Robert, Hutcheson, David, Strähle, Uwe
Keywords: Zebrafish, Chaperone, Co-chaperone, Muscle damage, hsp90a, UCS factor
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • DNA/genetics
  • Gene Expression Regulation, Developmental
  • HSP90 Heat-Shock Proteins/genetics
  • HSP90 Heat-Shock Proteins/metabolism*
  • Heart/embryology
  • In Vitro Techniques
  • Microscopy, Electron, Transmission
  • Molecular Sequence Data
  • Muscle Development/genetics
  • Muscle Development/physiology*
  • Muscle Proteins/genetics
  • Muscle Proteins/metabolism*
  • Muscle, Skeletal/embryology
  • Muscle, Skeletal/metabolism
  • Muscle, Skeletal/ultrastructure
  • Mutation
  • Myocardium/metabolism
  • Myocardium/ultrastructure
  • Phenotype
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 17586488 Full text @ Dev. Biol.
Contraction of muscles is mediated by highly organized arrays of myosin motor proteins. We report here the characterization of a mutation of a UCS gene named steif/unc-45b that is required for the formation of ordered myofibrils in both the skeletal and cardiac muscles of zebrafish. We show that Steif/Unc-45b interacts with the chaperone Hsp90a in vitro. The two genes are co-expressed in the skeletal musculature and knockdown of Hsp90a leads to impaired myofibril formation in the same manner as lack of Steif/Unc-45b activity. Transcripts of both genes are up-regulated in steif mutants suggesting co-regulation of the two genes. Our data indicate a requirement of Steif/unc-45b and Hsp90a for the assembly of the contractile apparatus in the vertebrate skeletal musculature.