|ZFIN ID: ZDB-PUB-070629-29|
The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis
Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U.
|Source:||Developmental Biology 308(1): 133-143 (Journal)|
|Registered Authors:||Behra, Martine, Etard, Christelle, Fischer, Nadine, Geisler, Robert, Hutcheson, David, Strähle, Uwe|
|Keywords:||Zebrafish, Chaperone, Co-chaperone, Muscle damage, hsp90a, UCS factor|
|PubMed:||17586488 Full text @ Dev. Biol.|
Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U. (2007) The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis. Developmental Biology. 308(1):133-143.
ABSTRACTContraction of muscles is mediated by highly organized arrays of myosin motor proteins. We report here the characterization of a mutation of a UCS gene named steif/unc-45b that is required for the formation of ordered myofibrils in both the skeletal and cardiac muscles of zebrafish. We show that Steif/Unc-45b interacts with the chaperone Hsp90a in vitro. The two genes are co-expressed in the skeletal musculature and knockdown of Hsp90a leads to impaired myofibril formation in the same manner as lack of Steif/Unc-45b activity. Transcripts of both genes are up-regulated in steif mutants suggesting co-regulation of the two genes. Our data indicate a requirement of Steif/unc-45b and Hsp90a for the assembly of the contractile apparatus in the vertebrate skeletal musculature.