PUBLICATION
Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio
- Authors
- Yang, H., Carmichael, C., Varga, Z.M., and Tiersch, T.R.
- ID
- ZDB-PUB-070625-6
- Date
- 2007
- Source
- Theriogenology 68(2): 128-136 (Journal)
- Registered Authors
- Carmichael, Carrie, Varga, Zoltan M.
- Keywords
- Sperm cryopreservation, In vitro fertilization, Zebrafish, Danio rerio
- MeSH Terms
-
- Animals
- Cryopreservation/methods*
- Cryopreservation/standards
- Cryoprotective Agents/toxicity
- Male
- Methanol/toxicity
- Osmolar Concentration
- Sperm Motility/drug effects
- Spermatozoa*/drug effects
- Temperature
- Zebrafish/physiology*
- PubMed
- 17544099 Full text @ Theriogenology
Citation
Yang, H., Carmichael, C., Varga, Z.M., and Tiersch, T.R. (2007) Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio. Theriogenology. 68(2):128-136.
Abstract
Sperm cryopreservation offers potential for long-term storage of genetic resources. However, the current protocols for zebrafish Danio rerio are cumbersome and poorly reproducible. Our objective was to facilitate adoption of cryopreservation by streamlining methods from sperm collection through thawing and use. First, sperm activation was evaluated, and motility was completely inhibited when osmolality of the extender was >/=295-300mOsmol/kg. To evaluate cryoprotectant toxicity, sperm were incubated with dimethyl sulfoxide (DMSO), N,N-dimethyl acetamide (DMA), methanol, or glycerol at 5, 10, and 15% concentrations. Based on motility, DMSO, DMA, and methanol (
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping