ZFIN ID: ZDB-PUB-070614-4
A Large-scale Mutagenesis Screen to Identify Seizure-resistant Zebrafish
Baraban, S.C., Dinday, M.T., Castro, P.A., Chege, S., Guyenet, S., and Taylor, M.R.
Date: 2007
Source: Epilepsia   48(6): 1151-1157 (Journal)
Registered Authors: Taylor, Michael
Keywords: none
MeSH Terms:
  • Animals
  • Behavior, Animal/drug effects
  • Behavior, Animal/physiology
  • Disease Models, Animal*
  • Genetic Predisposition to Disease/genetics
  • Genetic Testing*
  • Larva/genetics
  • Larva/physiology
  • Locomotion/drug effects
  • Locomotion/genetics
  • Mutagenesis/genetics*
  • Pentylenetetrazole/pharmacology
  • Seizures/chemically induced
  • Seizures/epidemiology
  • Seizures/prevention & control*
  • Status Epilepticus/chemically induced
  • Status Epilepticus/prevention & control
  • Superior Colliculi/physiology
  • Zebrafish/genetics*
PubMed: 17521353 Full text @ Epilepsia
Methods: Seizures were induced with pentylenetetrazole (PTZ). Zebrafish were analyzed between 3 and 7 days postfertilization (dpf). Genome mutations were induced in founders by using N-ethyl-nitrosourea (ENU). Seizure behavior was monitored by using a high-speed camera and quantified by locomotion-tracking software. Electrographic activity was monitored by using a field-recording electrode placed in the optic tectum of agar-immobilized zebrafish. Results: Short-term PTZ exposure elicited a burst-suppression seizure pattern in 3-dpf zebrafish and more complex activity consisting of interictal- and ictal-like discharges at 7 dpf. Prolonged exposure to PTZ induced status epilepticus-like seizure activity and fatality in wild-type zebrafish larvae. With a PTZ survival assay at 6-7 dpf, we identified six zebrafish mutants in a forward-genetic screen covering nearly 2,000 F(2) families. One mutant (s334) also was shown to exhibit reduced behavioral activity on short-term PTZ exposure and an inability to generate long-duration ictal-like discharge. Conclusions: Zebrafish offers a powerful tool for the identification and study of a genetic basis for seizure resistance.