PUBLICATION
A two-color acid-free cartilage and bone stain for zebrafish larvae
- Authors
- Walker, M., and Kimmel, C.
- ID
- ZDB-PUB-070523-23
- Date
- 2007
- Source
- Biotechnic & histochemistry : official publication of the Biological Stain Commission 82(1): 23-28 (Journal)
- Registered Authors
- Kimmel, Charles B., Walker, Macie B.
- Keywords
- alcian blue, alizarin red, PCR genotyping
- MeSH Terms
-
- Zebrafish
- Polymerase Chain Reaction
- Coloring Agents*
- Histocytochemistry/methods*
- Magnesium Chloride*
- Acids
- Bone and Bones/anatomy & histology*
- Cartilage/anatomy & histology*
- Larva/anatomy & histology*
- Animals
- PubMed
- 17510811 Full text @ Biotech. Histochem.
Citation
Walker, M., and Kimmel, C. (2007) A two-color acid-free cartilage and bone stain for zebrafish larvae. Biotechnic & histochemistry : official publication of the Biological Stain Commission. 82(1):23-28.
Abstract
Traditionally, cartilage is stained by alcian blue using acidic conditions to differentiate tissue staining. The acidic conditions are problematic when one wishes to stain the same specimen for mineralized bone with alizarin red, because acid demineralizes bone, which negatively affects bone staining. We have developed an acid-free method to stain cartilage and bone simultaneously in zebrafish larvae. This method has the additional advantage that PCR genotyping of stained specimens is possible.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping