PUBLICATION
A choice behavior for morphine reveals experience-dependent drug preference and underlying neural substrates in developing larval zebrafish
- Authors
- Bretaud, S., Li, Q., Lockwood, B.L., Kobayashi, K., Lin, E., and Guo, S.
- ID
- ZDB-PUB-070427-11
- Date
- 2007
- Source
- Neuroscience 146(3): 1109-1116 (Journal)
- Registered Authors
- Guo, Su, Li, Qiang
- Keywords
- choice behavior, zebrafish, reward, addiction, opioid system, dopamine system
- MeSH Terms
-
- Analgesics, Opioid/pharmacology*
- Animals
- Biogenic Amines/physiology
- Choice Behavior/drug effects*
- Chromatography, Liquid
- Cloning, Molecular
- DNA Mutational Analysis
- Dopamine/physiology
- Immunohistochemistry
- In Situ Hybridization
- Larva/physiology*
- Mass Spectrometry
- Morphine/pharmacology*
- Motor Activity/physiology
- Naloxone/pharmacology
- Narcotic Antagonists/pharmacology
- Nerve Net/physiology
- Neurotransmitter Agents/physiology
- Receptors, Odorant/genetics
- Receptors, Odorant/physiology
- Receptors, Opioid/physiology
- Receptors, Opioid, mu/genetics
- Receptors, Opioid, mu/physiology
- Reinforcement, Psychology
- Reverse Transcriptase Polymerase Chain Reaction
- Reward
- Signal Transduction/physiology
- Zebrafish/physiology*
- PubMed
- 17428610 Full text @ Neuroscience
Citation
Bretaud, S., Li, Q., Lockwood, B.L., Kobayashi, K., Lin, E., and Guo, S. (2007) A choice behavior for morphine reveals experience-dependent drug preference and underlying neural substrates in developing larval zebrafish. Neuroscience. 146(3):1109-1116.
Abstract
Transparent larval zebrafish offer the opportunity to unravel genetic and neuronal networks underlying behavior in a developing system. In this study, we developed a choice chamber paradigm to measure reward-associated behavior in larval zebrafish. In the chamber where larval zebrafish have a choice of spending their time in either a water- or morphine-containing compartment, larvae that have previously experienced morphine spend significantly more time in the compartment containing morphine. This behavior can be attentuated by pre-treatment with antagonists of the opioid receptor or the dopamine receptor, and furthermore, is impaired in the too few mutant, which has a genetic deficiency in the production of specific groups of dopaminergic and serotonergic neurons in the ventral forebrain. These results uncover a choice behavior for an addictive substance in larval zebrafish that is mediated through central opioid and monoaminergic neurotransmitter systems.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping