|ZFIN ID: ZDB-PUB-070409-22|
The disarrayed mutation results in cell cycle and neurogenesis defects during retinal development in zebrafish
Baye, L.M., and Link, B.A.
|Source:||BMC Developmental Biology 7(1): 28 (Journal)|
|Registered Authors:||Baye, Lisa, Link, Brian|
|PubMed:||17411431 Full text @ BMC Dev. Biol.|
Baye, L.M., and Link, B.A. (2007) The disarrayed mutation results in cell cycle and neurogenesis defects during retinal development in zebrafish. BMC Developmental Biology. 7(1):28.
ABSTRACTBACKGROUND: The vertebrate retina is derived from proliferative neuroepithelial cells of the optic cup. During retinal development, the cell cycle and process of neurogenesis are coordinated in neuroepithelial progenitor cells and previous analyses have demonstrated reciprocal influences between the cell cycle and neurogenesis. However the specific mechanisms and exact relationships of cell cycle regulation and neurogenesis in the retina remain largely unknown. RESULTS: We have isolated and characterized a zebrafish mutant, disarrayed (drya64), which exhibits retinal defects in cell cycle regulation and neurogenesis. By 42 hours post fertilization (hpf), disarrayed mutants show small eyes and a reduced forebrain. Other aspects of development appear normal. Although retinogenesis is delayed, mutant retina eventually show all major cell types and begin to differentiate. Examination of the disarrayed mitotic cycle using BrdU and direct imaging techniques revealed that retinal neuroepithelial cells have an extended cell cycle period and reduced rate of cell cycle exit (neurogenesis), despite the fact that neurogenesis initiates at the appropriate time of development. Genetic mosaic analyses indicate that the cell cycle phenotype of disarrayed is cell-non-autonomous. CONCLUSION: The disarrayed mutant shows defects in both cell cycle regulation and neurogenesis and provides insights into the coordinated regulation of these processes during retinal development.