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ZIRC
ZFIN ID: ZDB-PUB-070330-17
The zebrafish udu gene encodes a novel nuclear factor and is essential for primitive erythroid cell development
Liu, Y., Du, L., Osato, M., Teo, E.H., Qian, F., Jin, H., Zhen, F., Xu, J., Guo, L., Huang, H., Chen, J., Geisler, R., Jiang, Y.J., Peng, J., and Wen, Z.
Date: 2007
Source: Blood   110(1): 99-106 (Journal)
Registered Authors: Geisler, Robert, Jiang, Yun-Jin, Liu, Yanmei, Peng, Jinrong, Wen, Zilong
Keywords: none
MeSH Terms:
  • Animals
  • Cell Differentiation
  • Cell Proliferation
  • Erythrocytes, Abnormal/pathology
  • Erythroid Cells/cytology
  • Erythroid Precursor Cells/cytology*
  • Erythroid-Specific DNA-Binding Factors/genetics
  • Erythroid-Specific DNA-Binding Factors/physiology*
  • Erythropoiesis*
  • Mutation
  • Transcription Factors/physiology*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed: 17369489 Full text @ Blood
FIGURES
ABSTRACT
Hematopoiesis is a complex process which gives rise to all blood lineages in the course of an organism's lifespan. Yet the underlying molecular mechanism governing this process is not fully understood. Here we report the isolation and detailed study of a newly identified zebrafish ugly duckling (udu) mutant allele, udu(sq1). We show that loss-of-function mutation in the udu gene disrupts primitive erythroid cell proliferation and differentiation in a cell-autonomous manner, resulting in red blood cell (RBC) hypoplasia. Positional cloning reveals that the udu gene encodes a novel factor that contains two Paired Amphipathic alpha-Helix like (PAH-L) repeats and a putative SW13, ADA2, N-Cor and TFIIIB like (SANT-L) domain. We further show that the Udu protein is predominantly localized in the nucleus and deletion of the putative SANT-L domain abolishes its function. Our study indicates that the Udu protein is very likely to function as a transcription modulator essential for the proliferation and differentiation of erythroid lineage.
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