PUBLICATION
Zebrafish Mib and Mib2 Are Mutual E3 Ubiquitin Ligases with Common and Specific Delta Substrates
- Authors
- Zhang, C., Li, Q., and Jiang, Y.J.
- ID
- ZDB-PUB-070122-12
- Date
- 2007
- Source
- Journal of molecular biology 366(4): 1115-1128 (Journal)
- Registered Authors
- Jiang, Yun-Jin, Li, Qing, Zhang, Chengjin
- Keywords
- Mib homologs, Delta, Notch, E3 ligase, endocytosis
- MeSH Terms
-
- Amino Acid Sequence
- Ubiquitin/metabolism*
- Signal Transduction
- Intracellular Signaling Peptides and Proteins
- Animals
- Transfection
- Chlorocebus aethiops
- Substrate Specificity
- Molecular Sequence Data
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Protein Binding
- Ubiquitin-Protein Ligases/genetics
- Ubiquitin-Protein Ligases/metabolism*
- Zebrafish/genetics
- COS Cells
- Membrane Proteins/metabolism*
- Sequence Homology, Amino Acid
- PubMed
- 17196985 Full text @ J. Mol. Biol.
Citation
Zhang, C., Li, Q., and Jiang, Y.J. (2007) Zebrafish Mib and Mib2 Are Mutual E3 Ubiquitin Ligases with Common and Specific Delta Substrates. Journal of molecular biology. 366(4):1115-1128.
Abstract
It was already known that both mind bomb (mib) and mind bomb-2 (mib2) encode E3 ubiquitin ligases that target Delta in Notch activation. Here we further demonstrated that zebrafish Mib and Mib2, similar to their mouse orthologs, have a C-terminal-most RING finger-dependent E3 ubiquitin ligase activity. Mib and Mib2 are reciprocal E3 ubiquitin ligases and substrates. They function similarly in Notch signaling by using DeltaC as a common substrate. However, Mib2 behaves differently from Mib in DeltaD internalization. In addition, Mib and Mib2 bind differently to extracellular and intracellular parts of DeltaA and DeltaC. Finally, mutant Mibs, Mib(ta52b) with a missense mutation in the C-terminal-most RING finger (M1013R) and Mib(m132) with a premature stop codon that leads to a deletion of three RING fingers (C785stop), act dominant-negatively and compete with Mib2 in DeltaC ubiquitylation and internalization, suggesting a molecular basis for the antimorphic phenotypes (stronger than the null phenotypes) observed in zebrafish mib(ta52b) and mib(m132) alleles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping