PUBLICATION
Pluripotency and Chimera Competence of an Embryonic Stem Cell Line from the Sea Perch (Lateolabrax japonicus)
- Authors
- Chen, S.L., Sha, Z.X., Ye, H.Q., Liu, Y., Tian, Y.S., Hong, Y., and Tang, Q.S.
- ID
- ZDB-PUB-061227-13
- Date
- 2007
- Source
- Marine biotechnology (New York, N.Y.) 9(1): 82-91 (Journal)
- Registered Authors
- Keywords
- Cell transplantation, chimera, embryonic stem cells, pluripotency, sea perch, zebrafish
- MeSH Terms
-
- Animals
- Cell Differentiation/physiology*
- Cell Line
- Chimera/physiology*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/physiology*
- Embryonic Stem Cells/cytology
- Embryonic Stem Cells/physiology*
- Green Fluorescent Proteins/biosynthesis
- Green Fluorescent Proteins/genetics
- Microscopy, Fluorescence
- Perches/embryology*
- Perches/physiology*
- Transfection/veterinary
- Zebrafish/embryology
- PubMed
- 17136469 Full text @ Mar. Biotechnol.
Citation
Chen, S.L., Sha, Z.X., Ye, H.Q., Liu, Y., Tian, Y.S., Hong, Y., and Tang, Q.S. (2007) Pluripotency and Chimera Competence of an Embryonic Stem Cell Line from the Sea Perch (Lateolabrax japonicus). Marine biotechnology (New York, N.Y.). 9(1):82-91.
Abstract
A stable GFP-expressing (GFP(+)LJES1) cell strain was developed from the LJES1 cells obtained from sea perch (Lateolabrax japonicus,) embryos. GFP(+)LJES1 cells were induced in vitro by RA to differentiate into a variety of cell types and also had the ability to form embryoid body-like structures in suspension culture. To determine the differentiation potential of LJES1 cells in vivo, GFP(+)LJES1 cells were transplanted into sea perch and zebrafish embryos at mid-blastula stage. Twenty out of 478 transplanted sea perch embryos contained GFP-expressing LJES1 cells 24 h after microinjection. Fifteen chimera embryos developed into fry. In these chimeras, the GFP(+)LJES1 cells contributed to a variety of tissues including the head and trunk. In zebrafish, 221 embryos were microinjected with GFP(+)LJES1 cells and 22 chimera embryos and fries expressing GFP were obtained. Donor GFP(+)LJES1 cells contributed to various tissues in head and trunk of zebrafish embryos and hatched fry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping