PUBLICATION

The zebrafish genome contains two inducible, functional cyclooxygenase-2 genes

Authors
Ishikawa, T.O., Griffin, K.J., Banerjee, U., and Herschman, H.R.
ID
ZDB-PUB-061205-4
Date
2007
Source
Biochemical and Biophysical Research Communications   352(1): 181-187 (Journal)
Registered Authors
Griffin, Kevin
Keywords
none
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • COS Cells
  • Cell Line
  • Chlorocebus aethiops
  • Conserved Sequence
  • Cyclooxygenase 2/chemistry
  • Cyclooxygenase 2/genetics*
  • Cyclooxygenase 2/metabolism*
  • Genome/genetics*
  • Isoenzymes/chemistry
  • Isoenzymes/genetics
  • Isoenzymes/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Zebrafish/genetics*
PubMed
17112475 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Cyclooxygenase is a key enzyme in prostanoid biosynthesis. Mammalian species have two cyclooxygenases, constitutively expressed cyclooxygenase-1 (Cox-1) and inducible cyclooxygenase-2 (Cox-2). Cox-1 and/or Cox-2 have been also identified in other vertebrates, including fish. We identified a second zebrafish Cox-2 gene orthologue, Cox-2b. All of the functionally important amino acids for cyclooxygenase enzymes are conserved in Cox-2b. The 3' untranslated region of the Cox-2b message contains AU rich elements characteristic of regulation at the level of mRNA stability. Constitutive tissue expression patterns for Cox-2a and Cox-2b are distinct, but overlap. Both Cox-2a and Cox-2b expression are inducible in the kidney when fish are exposed to tetradecanoylphorbol acetate. Like Cox-2a, Cox-2b protein, expressed in COS cells is functionally active. Thus, the zebrafish genome contains two functional, inducible Cox-2 genes. Database searching demonstrates that some fish genomes contain multiple Cox-1 or Cox-2 cyclooxygenase genes, suggesting alternate duplication and retention of this gene.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping