PUBLICATION
V(D)J recombination in zebrafish: Normal joining products with accumulation of unresolved coding ends and deleted signal ends
- Authors
- Li, Z., and Chang, Y.
- ID
- ZDB-PUB-061010-7
- Date
- 2007
- Source
- Molecular immunology 44(7): 1804-1813 (Journal)
- Registered Authors
- Li, Zhi
- Keywords
- Non-homologous end joining, Recombination ends, Zebrafish
- MeSH Terms
-
- Animals
- Base Sequence
- Gene Rearrangement, B-Lymphocyte, Heavy Chain/genetics*
- Gene Rearrangement, B-Lymphocyte, Light Chain/genetics*
- Molecular Sequence Data
- Recombination, Genetic
- VDJ Recombinases/metabolism
- Zebrafish/genetics*
- Zebrafish/immunology
- PubMed
- 17005252 Full text @ Mol. Immunol.
Citation
Li, Z., and Chang, Y. (2007) V(D)J recombination in zebrafish: Normal joining products with accumulation of unresolved coding ends and deleted signal ends. Molecular immunology. 44(7):1804-1813.
Abstract
V(D)J recombination proceeds from a site-specific cleavage to an imprecise end joining, via generation and resolution of recombination ends. Although rearranged antigen receptor genes isolated from zebrafish (Danio rerio) resemble those made in mammals, differences may arise during evolution from lower to higher vertebrates, in regard to efficiency, fidelity and regulation of this recombination. To elucidate the V(D)J recombination reaction in zebrafish, we characterized recombination ends transiently produced by zebrafish lymphocytes, as well as joining products. Similar to their mammalian counterpart, zebrafish lymphocytes make perfect signal joints and normal coding joints, indicating their competent end resolution machinery. However, recombination ends recovered from the same zebrafish lymphoid tissues exhibit some features that are not readily seen in normal mammalian counterpart: deleted signal ends and accumulation of opened coding ends. These results indicate that the recombination reaction in zebrafish lymphocytes is inefficient and less stringently regulated, which may result from unstable post-cleavage complexes, and/or slow transition from cleavage to resolution. Our data suggests that the V(D)J recombination machinery may have undergone evolution selection to become more efficient in higher jawed vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping