PUBLICATION

Nyctalopin is essential for synaptic transmission in the cone dominated zebrafish retina

Authors
Bahadori, R., Biehlmaier, O., Zeitz, C., Labhart, T., Makhankov, Y.V., Forster, U., Gesemann, M., Berger, W., and Neuhauss, S.C.
ID
ZDB-PUB-061010-6
Date
2006
Source
The European journal of neuroscience   24(6): 1664-1674 (Journal)
Registered Authors
Bahadori, Ronja, Biehlmaier, Oliver, Neuhauss, Stephan
Keywords
none
MeSH Terms
  • Animals
  • Cloning, Molecular/methods
  • Electroretinography/methods
  • Humans
  • Immunohistochemistry/methods
  • In Situ Hybridization/methods
  • Intracellular Signaling Peptides and Proteins/metabolism
  • Membrane Glycoproteins/genetics
  • Membrane Glycoproteins/metabolism
  • Morpholines/antagonists & inhibitors
  • Morpholines/chemistry
  • Nystagmus, Optokinetic/drug effects
  • Nystagmus, Optokinetic/physiology
  • Oligodeoxyribonucleotides, Antisense/pharmacology
  • Proteoglycans/genetics
  • Proteoglycans/metabolism*
  • Retina/cytology*
  • Retinal Cone Photoreceptor Cells/drug effects
  • Retinal Cone Photoreceptor Cells/metabolism*
  • Sequence Analysis, Protein
  • Synaptic Transmission/physiology*
  • Transfection/methods
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
17004930 Full text @ Eur. J. Neurosci.
Abstract
The first synapse in the vertebrate visual system is the photoreceptor synapse between rod and cone photoreceptors and the second-order bipolar cells. Although mutations in the nyctalopin gene (NYX) in humans lead to congenital stationary night blindness (CSNB1), affecting synaptic transmission between both types of photoreceptors and ON-bipolar cells, the function of nyctalopin in cone-dominant animal models has not been studied. Because the larval zebrafish retina is cone-dominant, we isolated the zebrafish nyx ortholog and raised a polyclonal antibody against the protein. Nyctalopin is expressed postsynaptically in both synaptic layers of the retina. Functional disruption via morpholino antisense injection leads to characteristic defects in the electroretinogram and defects in visual contrast sensitivity. We therefore demonstrated that nyctalopin plays a similar role in retinal synapse function in the cone pathway as in the rod pathway, thereby creating a genetic model for CSNB1 and its effects on cone vision.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes