PUBLICATION
Molecular structure and expression patterns of flounder (Paralichthys olivaceus) Myf-5, a myogenic regulatory factor
- Authors
- Tan, X., Zhang, Y., Zhang, P.J., Xu, P., and Xu, Y.
- ID
- ZDB-PUB-060921-5
- Date
- 2006
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 145(2): 204-213 (Journal)
- Registered Authors
- Zhang, Yuqing
- Keywords
- Flounder, Muscle, Myf-5, Myogenesis, Somites, Promoter, E-box, MyoD, Intron regulatory element
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Embryo, Nonmammalian/metabolism
- Fish Proteins/genetics*
- Fish Proteins/metabolism
- Flounder/embryology
- Flounder/genetics*
- Flounder/metabolism
- Gene Expression
- Introns
- Molecular Sequence Data
- Myogenic Regulatory Factor 5/genetics*
- Myogenic Regulatory Factor 5/metabolism
- Promoter Regions, Genetic
- PubMed
- 16963299 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Tan, X., Zhang, Y., Zhang, P.J., Xu, P., and Xu, Y. (2006) Molecular structure and expression patterns of flounder (Paralichthys olivaceus) Myf-5, a myogenic regulatory factor. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 145(2):204-213.
Abstract
Myf-5, a member of the myogenic regulatory factors (MRF), has been shown to be expressed in muscle precursors in early stage zebrafish embryos. The MRFs, including MyoD, Myf-5, Myogenin and MRF4, belong to the basic Helix-Loop-Helix transcription factors that contain a conserved basic Helix-Loop-Helix (bHLH) domain. To better understand the role of Myf-5 in the development of fish muscles, we have isolated the Myf-5 genomic sequence and cDNA from Flounder (Paralichthys olivaceus), and analyzed its structures and patterns of expression. Promoter analysis identified several putative transcription factor binding sites such as an E-box, NF-Y sites that might confer muscle-specific expression. Myf-5 transcripts were first detected in the paraxial mesoderm that gives rise to slow muscles. During somitogenesis, Myf-5 expression was found in developing somites. Myf-5 expression decreased gradually in somites in the anterior region, but remained strong in the newly formed somites. In the hatching stage, the expression was also detected in other muscle cells such as head muscle and fin muscle. In the growing fish, RT-PCR results showed that Myf-5 was expressed in the skeletal muscle and intestine.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping