PUBLICATION
Effects of chronic ethanol administration on brain protein levels: A proteomic investigation using 2-D DIGE system
- Authors
- Damodaran, S., Dlugos, C.A., Wood, T.D., and Rabin, R.A.
- ID
- ZDB-PUB-060921-20
- Date
- 2006
- Source
- European Journal of Pharmacology 547(1-3): 75-82 (Journal)
- Registered Authors
- Dlugos, Cynthia A., Rabin, Richard
- Keywords
- Ethanol, Proteomics, DIGE, (Zebrafish)
- MeSH Terms
-
- Animals
- Behavior, Animal/drug effects
- Brain/drug effects*
- Brain/metabolism
- Central Nervous System Depressants/pharmacology
- Electrophoresis, Gel, Two-Dimensional/methods
- Ethanol/pharmacology*
- Proteomics/methods*
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- Swimming/physiology
- Time Factors
- Zebrafish
- Zebrafish Proteins/analysis*
- PubMed
- 16978605 Full text @ Eur. J. Pharmacol.
Citation
Damodaran, S., Dlugos, C.A., Wood, T.D., and Rabin, R.A. (2006) Effects of chronic ethanol administration on brain protein levels: A proteomic investigation using 2-D DIGE system. European Journal of Pharmacology. 547(1-3):75-82.
Abstract
The effects of chronic ethanol treatment on the brain proteome were investigated in the long-fin striped strain of zebrafish Danio rerio. Prolonged exposure to 0.5% (v/v) ethanol resulted in the development of tolerance to the ethanol-induced disruption of normal swimming behavior. This behavioral tolerance was manifested after two weeks of continuous treatment and was maintained for an additional three weeks. After four weeks of ethanol treatment, zebrafish brains were divided into 40,000 g supernatant and pellet fractions, and an Ettan 2-D fluorescence difference gel electrophoresis (DIGE) system was used to detect ethanol-induced alterations in the level of protein expression. Protein identification was carried out using matrix assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry and the Mascot and ProFound search engines. In the present study, we have identified some novel protein targets as well as substantiated some putative previous targets of chronic ethanol exposure.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping