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ZFIN ID: ZDB-PUB-060906-11
Dual roles for adenomatous polyposis coli in regulating retinoic acid biosynthesis and Wnt during ocular development
Nadauld, L.D., Chidester, S., Shelton, D.N., Rai, K., Broadbent, T., Sandoval, I.T., Peterson, P.W., Manos, E.J., Ireland, C.M., Yost, H.J., and Jones, D.A.
Date: 2006
Source: Proceedings of the National Academy of Sciences of the United States of America   103(36): 13409-13414 (Journal)
Registered Authors: Broadbent, Talmage, Yost, H. Joseph
Keywords: colon cancer, retina, APC
MeSH Terms:
  • Adenomatous Polyposis Coli Protein/deficiency
  • Adenomatous Polyposis Coli Protein/genetics
  • Adenomatous Polyposis Coli Protein/metabolism*
  • Animals
  • Eye/drug effects
  • Eye/embryology*
  • Gene Expression Regulation, Developmental
  • Homozygote
  • Microinjections
  • Molecular Sequence Data
  • Mutation
  • Oligonucleotides, Antisense/pharmacology
  • Tretinoin/metabolism*
  • Tretinoin/pharmacology
  • Wnt Proteins/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
PubMed: 16938888 Full text @ Proc. Natl. Acad. Sci. USA
Congenital hypertrophy/hyperplasia of the retinal pigmented epithelium is an ocular lesion found in patients harboring mutations in the adenomatous polyposis coli (APC) tumor suppressor gene. We report that Apc-deficient zebrafish display developmental abnormalities of both the lens and retina. Injection of dominant-negative Lef reduced Wnt signaling in the lens but did not rescue retinal differentiation defects. In contrast, treatment of apc mutants with all-trans retinoic acid rescued retinal differentiation defects but had no apparent effect on the lens. We identified Rdh5 as a retina-specific retinol dehydrogenase controlled by APC. Morpholino knockdown of Rdh5 phenocopied the apc mutant retinal differentiation defects and was rescued by treatment with exogenous all-trans retinoic acid. Microarray analyses of apc mutants and Rdh5 morphants revealed a profound overlap in the transcriptional profile of these embryos. These findings support a model wherein Apc serves a dual role in regulating Wnt and retinoic acid signaling within the eye and suggest retinoic acid deficiency as an explanation for APC mutation-associated retinal defects such as congenital hypertrophy/hyperplasia of the retinal pigmented epithelium.