PUBLICATION
Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish
- Authors
- Eroglu, B., Wang, G., Tu, N., Sun, X., and Mivechi, N.F.
- ID
- ZDB-PUB-060816-18
- Date
- 2006
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 235(10): 2722-2735 (Journal)
- Registered Authors
- Keywords
- zebrafish, Brg1 mutant (yng), morpholino, neurogenesis, neural crest
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing
- Animals
- Cell Differentiation/genetics
- Cell Differentiation/physiology
- Chromatin/metabolism
- Chromatin Immunoprecipitation
- Chromosomal Proteins, Non-Histone/genetics
- Chromosomal Proteins, Non-Histone/metabolism
- Chromosomal Proteins, Non-Histone/physiology*
- DNA Helicases/genetics
- DNA Helicases/metabolism
- DNA Helicases/physiology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental/genetics
- Immunohistochemistry
- In Situ Hybridization
- Mutation/genetics
- Neural Crest/embryology*
- Neural Crest/metabolism
- Organogenesis/genetics
- Organogenesis/physiology
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Time Factors
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Transcription Factors/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology*
- PubMed
- 16894598 Full text @ Dev. Dyn.
Citation
Eroglu, B., Wang, G., Tu, N., Sun, X., and Mivechi, N.F. (2006) Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(10):2722-2735.
Abstract
Brg1 is a member of the SWI/SNF chromatin-remodeling complex, and in some organisms Brg1 has been shown to interact with beta-catenin and positively control the TCF/LEF transcription factor that is located downstream of the Wnt signal transduction pathway. During development, TCF/LEF activity is critical during neurogenesis and head induction. In zebrafish, Brg1-deficient embryos exhibit retinal cell differentiation and eye defects; however, the role of Brg1 in neurogenesis and neural crest cell induction remains elusive. We used zebrafish deficient in Brg1 (yng) or Brg1 specific-morpholino oligonucleotide-mediated knockdown to analyze the embryonic requirements of Brg1. Our results indicate that reduction in Brg1 expression leads to the expansion of the forebrain-specific transcription factor, six3, and marked reduction in expression of the mid/hind-brain boundary and hind-brain genes, engrailed2 and krox20, respectively. At 12 hpf, the expression of neural crest specifiers are severely affected in Brg1-morpholino-injected embryos. These results suggest that Brg1 is involved in neural crest induction, which is critical for the development of neurons, glia, pigment cells, and craniofacial structures. Brg1 is a maternal factor, and brg1-deficient embryos bearing the yng mutation derived from heterozygote intercrosses exhibit lesser effects on neural crest-specific gene expression, but show defects in neurogenesis and neural crest cell differentiation. This is exhibited by the aberrant brain patterning, a reduction in the sensory neurons, and craniofacial defects. These results further elucidate the critical role for Brg1 in neurogenesis, neural crest induction, and differentiation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping