PUBLICATION
Modified bacterial artificial chromosomes for zebrafish transgenesis
- Authors
- Yang, Z., Jiang, H., Chachainasakul, T., Gong, S., Yang, X.W., Heintz, N., and Lin, S.
- ID
- ZDB-PUB-060719-9
- Date
- 2006
- Source
- Methods (San Diego, Calif.) 39(3): 183-188 (Journal)
- Registered Authors
- Jiang, Hong, Lin, Shuo, Yang, Zhongan
- Keywords
- Transgenic zebrafish, GFP, RFP, Modification, Zebrafish BAC and PAC
- MeSH Terms
-
- Recombination, Genetic
- Zebrafish/embryology
- Zebrafish/genetics*
- Green Fluorescent Proteins/analysis
- Rec A Recombinases/genetics
- Luminescent Proteins/analysis
- Escherichia coli/genetics
- Recombinant Fusion Proteins/analysis
- Animals, Genetically Modified/genetics*
- Embryo, Nonmammalian
- Plasmids/genetics
- Genes, Reporter
- Genes, Bacterial
- Genetic Engineering/methods*
- Gene Expression Regulation
- Animals
- Chromosomes, Artificial, Bacterial/genetics*
- PubMed
- 16828309 Full text @ Methods
Citation
Yang, Z., Jiang, H., Chachainasakul, T., Gong, S., Yang, X.W., Heintz, N., and Lin, S. (2006) Modified bacterial artificial chromosomes for zebrafish transgenesis. Methods (San Diego, Calif.). 39(3):183-188.
Abstract
Transgenesis using bacterial artificial chromosomes (BAC) offers greater fidelity in directing desirable expression of foreign genes. Application of this technology in the optically transparent zebrafish with fluorescent protein reporters enables unparalleled visual analysis of regulation of gene expression in a living organism. Here we describe a streamlined procedure of direct selecting multiple BAC clones based on public sequence databases followed by rapid modification with GFP or RFP for transgenic analysis in zebrafish. Experimental procedures for BAC DNA preparation, microinjection of zebrafish embryos and screening of transgenic zebrafish carrying GFP/RFP modified BAC clones are detailed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping